兩階段培養及巴克素對孤挺花組培苗生長與發根之影響

Effects of Two-Stage Culture and Paclobutrazol on In Vitro Bulblet Growth and Rooting of Hippeastrum hybridum

陳威臣(Uei-Chern Chen) ； 吳姿穎(Tzu-Ying Wu) ； 曹進義(Jhin-Yi Tsao) ； 夏奇鈮(Chi-Ni Hsia)

《台灣農業研究》 67卷1期 (2018/03) Pp. 44-53

https://doi.org/10.6156/JTAR.201803_67(1).0005

摘要

本研究利用孤挺花(Hippeastrum hybridum Hort.)「台農1號-紅粉佳人」('Tainung No.1-Pink Lady')、「紅獅」('Red Lion')及「千禧之星」('Blossom Peacock')組培苗為材料，探討兩階段培養及巴克素對鱗莖生長與發根之影響。「台農1號」鱗莖(直徑約5 mm)於含有0.1 mg L^(-1)苯甲基腺嘌呤(benzylaminopurine; BA)與0.1 mg L^(-1)奈乙酸(α-naphthalene acetic acid; NAA)之液態或固態MS培養基中培養8 wk(第一階段)，再接種至相同組成之固態培養基8 wk後(第二階段)。結果顯示，液-固態兩階段培養具有顯著增加組培苗鮮重、葉數、葉長及根數之效果。利用「紅獅」與「千禧之星」鱗莖(直徑約10 mm)進行巴克素(Paclobutrazol; PBZ)液態培養試驗，結果顯示，對照組鱗莖培養至8 wk時，已有部分組培苗出現嚴重褐化的現象，建議繼代培養間隔時間不宜超過8 wk。「紅獅」組培鱗莖在50-75 mg L^(-1) PBZ培養條件下，PBZ處理之根數顯著較多於對照組，但PBZ處理間並無顯著差異。然而，「千禧之星」鱗莖在25-75 mg L^(-1) PBZ處理時根系生長受到抑制，且濃度越高時根數就越少，而75 mg L^(-1) PBZ處理則較對照組具有顯著較短與較寬的葉片；將PBZ濃度降低至5 mg L^(-1)，則有增大鱗莖與促進發根之效果。

關鍵字

朱頂紅 ； 液-固態培養 ； 生長抑制劑 ； 多效唑

並列摘要

An improved method for in vitro bulblet growth and rooting of Hippeastrum hybridum was established in this study. In vitro bulblets of Hippeastrum hybridum 'Tainung No. 1', 'Red Lion', and 'Blossom Peacock' were used as materials to conduct the liquid-solid or solid-solid two-stage culture, and additional paclobutrazol was added into culture medium to enhance growth and rooting of in vitro Amaryllis bulblets. In vitro bulbs (5 mm diameter) of 'Tainung No. 1' were cultured in liquid or solid MS medium containing 1 mg L^(-1) BA and 0.1 mg L^(-1) NAA for 8 wk of culture before transferring to a solid MS medium with the same components for another 8 wk of culture. The results showed that liquid- solid culture was superior to solid-solid culture on fresh weight of bulblets, leaf number, leaf length, and root number. In vitro bulblets of 'Red Lion' and 'Blossom Peacock' showed severe browning or death after 8 wk of culture on the control medium. Therefore, it is suggested that the subculture interval should not exceed 8 wks. The root number of 'Red Lion' bulblets by 50-75 mg L^(-1) PBZ treatment was found significantly higher than that of control. No significant difference among PBZ treatments was found. However, root numbers of 'Blossom Peacock' were reduced treated with 25-75 mg L^(-1) PBZ concentrations. Leaf with shorter and wider shape was observed in 75 mg L^(-1) PBZ treatment. However, a lower concentration of 5 mg L^(-1) PBZ was found beneficially to bulb diameter, root number and root length.

並列關鍵字

Amaryllis ； Liquid-solid culture ； Plant growth retardant ； PP333