Weeds are estimated to cause more than $40 billion in annual global losses through degraded agricultural and silvicultural productivity. Manual removal, mechanical cultivation, cultural practices, or chemical herbicides can control weeds. However, use of physical and/or chemical methods of weed control alone is not feasible, desirable, or sufficient in every situation. The application of bacteria, fungi and viruses to achieving this goal has received increasingly great attention over the last three decades. Using mycoherbicides for control of weeds has great potential. The fungi with herbicidal activity are mostly focus on the fungus colletotrichum, Phoma, Sclerotinia and other six fungi. Current constrains in the development of mycoherbicides were analysed, and approached to overcome the limitations in future were summarized.