Diversity of spider species in an ecological system is an important indicator. Spiders act as natural predators of many insects and small animals. They are also crucial source of energy for birds and many other predators. Information from investigation of diversity of spider helps people realizing its richness in a specific region. The Heshe Nature Education Area, NTU Experimental Forests, located in Xinyi Township, Nantou County, at about 780 meters above sea level, has been planted with a range of tree specimen. To understand diversity of spider species in the area, 8 surveys were conducted in 2015. Because of altered behaviors of different spider species throughout the day, the survey was conducted once a month in daytime and nighttime, respectively. A total of 19 species within 6 families were recorded during the daytime surveys. On the contrary, there were 23 species within 6 families identified during the nighttime surveys. This study was the first to provide a list of spider species in the Heshe area, and is made available for future study and education.