This study examined how to reduce injuries caused by sharp instruments during cleaning in medical contexts. Injuries resulting from sharp instruments can increase the risk of infection and may even be fatal. The average number of injuries recorded before the study was 1.125 per month. Following analysis, we determined that excessive dryness, lack of safety equipment, lack of regular training, failure to follow procedure, and provision of imperfect guidelines were responsible for the high rate of injury caused when cleaning sharp instruments. We employed decision matrix analysis to evaluate the reasons behind the injuries and proposed the following solutions: implementing training programs, purchasing relevant equipment, standardizing operating procedures, and revising cleaning guidelines. Following implementation of the solutions, the average number of injuries caused by sharp instrument during cleaning decreased from 1.125 to 0.05 per month, improving overall workplace safety.