鹽類濃度、蔗糖、植物生長素及培養容器瓶口覆蓋物對高氏柴胡組培苗發根與馴化之影響

Influence of Salt Strength, Sucrose, Auxins and Container Closure on Root Formation and Acclimation of in Vitro Bupleurum kaoi Plantlets

陳威臣(Uei-Chern Chen) ； 夏奇鈮(Chi-Ni Hsia) ； 葉茂生(Mau-Shing Yeh) ； 蔡新聲(Hsin-Sheng Tsay)

《中華農業研究》 53卷4期 (2004/12) Pp. 249-260

https://doi.org/10.29951/JARC.200412.0004

摘要

本研究探討培養基組成中之MS (Murashige & Skoog)鹽類濃度、蔗糖濃度與植物生長素，對高氏柴胡(Bupleurum kaoi Liu, Chao et Chuang)組培苗發根之影響，並藉由瓶口覆蓋物處理降低瓶內溼度，以提高瓶苗移植存活率之方法，建立高氏柴胡組織培養之大量繁殖種苗體系。結果顯示，低MS鹽類濃度（1/4MS與1/2MS）及3%蔗糖培養基之組培苗根系形成較佳且存活率較高。組培苗培養於含有0.5 mg/l吲（口朶）丁酸(indole-3-butyric acid; IBA)之1/2MS培養基，發根率可達100%，且組培苗不會產生基部癒合組織化現象。利用鋁箔紙覆蓋培養2-3週後，再以三層藥包紙進行覆蓋物置換處理之瓶苗與根系均生長良好，瓶苗移植存活率約為76%，較高於鋁箔紙覆蓋培養6週之處理（約33%）。將組培苗培養於含0.5 mg/l IBA與0.1-0.2 mg/l奈乙酸(α-naphthaleneacetic acid; NAA)之1/2MS培養基，於培養2週後再進行藥包紙置換處理，可形成具有較粗根徑之組培苗，將出瓶組培苗除去部分葉片後剩餘長約3-4 cm之瓶苗，移植於已滅菌之培養土(BioMix)：蛭石：珍珠石＝1:1:1 (v/v)混合介質，育苗盤放入有蓋透明塑膠盒後置於日夜溫為22℃(10 h)/18℃(14 h)、光照（約60μmol/平方公尺/s）之生長箱進行馴化處理，4週後之存活率高達92%，顯示添加少量NAA有利於高氏柴胡組培苗之發根與移植存活率的提昇。本研究利用組培苗瓶內處理(改善培養基組成與培養容器瓶口覆蓋物)及出瓶後馴化處理(有蓋透明塑膠盒)之結果，將有助於高氏柴胡組培苗產業化生產系統之建立。

關鍵字

高氏柴胡 ； 藥用植物 ； 發根 ； 馴化 ； 培養容器瓶口覆蓋物

並列摘要

An efficient protocol for producing high quality rooted plantlets which are easy to acclimatize for field growth was established for micropropagation of Bupleurum kaoi Liu, Chao et Chuang. In this study, the medium containing half- and quarter- strength of MS basal salts (1/2MS and 1/4MS) and 3% (w/v) sucrose was found suitable for shoot survival and root formation of in vitro B. kaoi plantlets. A 100% root formation rate was obtained from in vitro shoots cultured on the medium containing half-strength MS salt, 0.5 mg/l indole-3-butyric acid (IBA) without basal callus further induced. An increased survival rate for in vitro plantlets acclimation by using ventilative container closure of dispense paper to exchange the normal used aluminum foil after 2-3 weeks culturing in a total 6 weeks incubation span was achieved. Leaves of in vitro rooted plantlets were partial trimmed (3-4 cm remnant in length) and transplanted into BioMix: vermiculite: perlite = 1: 1: 1 (v/v) mixed substrate inside a transparent plastic box with cover. Boxes were put into a growth chamber under 22℃ (14 h in light)/18℃ (10 h in darkness) environmental condition for 4 weeks acclimation. The best survival rate was 92% after 4 weeks of acclimation from plantlets previously cultured on half-strength MS medium containing with 0.5 mg/l IBA and 0.1-0.2 mg/lα -naphthaleneacetic acid (NAA), in combination with changing aluminum foil with 3 layers of dispense paper as container closure after 2 weeks culturing. These results including in vitro treatment by modifying medium components and exchanging container closure for root formation and plantlets quality; ex vitro treatment by planting plantlets inside a transparent plastic box with cover for reducing transpiration would be useful for industrial production of in vitro Bupleurum kaoi plantlets.

並列關鍵字

Bupleurum kaoi ； Medicinal plant ； Rooting ； Acclimation ； Container closure