Tuberose is one of the most important cut flowers in Taiwan. It has also been regarded to have great potential for becoming a promising floral crop recently However, only two major cultivars 'Single' and 'Double' are cultivated for commercial production in Taiwan. The less variations of flower shape, fewer size and flower color in tuberose cause people pay more urgent to breed ness cultivars Polianthes tuberose 'Single', P. tuber-use 'Double', P.×howardii, P.×blissii and several selected hybrids are collected for this breeding program Hybridization and buck-crossing breeding were made among these collected cultivars and selected hybrids. Fifteen clones with improved characteristics are reselected, including three clones of while, sin clones of pink-red, four clones of purple-red, and two clones of yellow flower color.