Plasma glutamine and cystine are decreased and negatively correlated with endomysial antibody in children with celiac disease

腹腔疾病儿童血浆谷氨酰胺和胱氨酸水平下降与肌内膜抗体有关

Eylem Sevinc ； Nergiz Sevinc ； Himmet Haluk Akar ； Banu Demet Ozelcoskun ； Gülten Can Sezgin ； Duran Arslan ； Mustafa Kendirci

《Asia Pacific Journal of Clinical Nutrition》 25卷3期 (2016/09) Pp. 452-456

https://doi.org/10.6133/apjcn.092015.09

摘要

背景与目的：谷氨酰胺是一种非必需氨基酸，具有提高肠黏膜再生和吸收的功能。谷胱甘肽是人体一种具有抗氧化的重要分子，由胱氨酸合成。该研究的主要目的是检测腹腔疾病（CD）患儿血浆谷氨酰胺和胱氨酸水平，并与对照组比较。其次是探讨这些氨基酸与肌内膜抗体（EMA）是否相关。方法与研究设计：选择50名CD患儿，另选年龄和性别相匹配的50名健康儿童作为对照。采用串联质谱法测定儿童血浆谷氨酰胺和胱氨酸的水平。结果：CD患儿血浆谷氨酰胺（808 vs 870 μmol/L）和胱氨酸水平（19 vs 48.5 μmol/L）显著低于对照组儿童（p＜0.05，EMA 阳性的患儿血浆谷氨酰胺（797 vs 928μmol/L, n=42）和胱氨酸水平（18 vs 31.5 μmol/L, n=8）显著低于EMA 阴性的CD 患儿。我们未发现EMA 阴性的CD 患儿和健康对照组儿童血浆谷氨酰胺（928 vs 870 μmol/L）及胱氨酸水平（31.5 vs 48.5 μmol/L）之间存在差异（p＞0.05）。血清EMA 与血浆胱氨酸水平（r=-0,321, p=0.023）和谷氨酰胺（r=-0.413, p=0.003）呈显著负相关。结论：该研究表明，CD患儿血浆谷氨酰胺和胱氨酸含量明显低于健康对照组。另外，这些氨基酸的含量与EMA水平呈显著负相关。

關鍵字

胱氨酸 ； 谷氨酰胺 ； 腹腔疾病 ； 肌内膜抗体

並列摘要

Background and Objectives: Glutamine is a nonessential amino acid which improves intestinal mucosal regeneration and absorption. Glutathione is a vital molecule for antioxidant reactions and is synthesized from cystine. The first aim of the study is to measure the plasma glutamine and cystine in children with celiac disease (CD) and compare them with controls. The second aim of this study is to investigate whether these amino acids are correlated with endomysial antibody (EMA) or not. Methods and Study Design: Fifty children with CD were compared to 50 healthy, age, and sex matched normal children as control. Plasma glutamine and cystine levels of the children were measured by using tandem mass spectrometry. Results: Plasma glutamine (808 vs 870 μmol/L) and cystine (19 vs 48.5 μmol/L) were significantly lower in the celiac group than the controls (p＜0.05). The levels of plasma glutamine (797 vs 928 μmol/L, n=42) and cystine (18 vs 31.5 μmol/L, n=8) were lower (p＜0.05) in the EMA-positive than the EMA-negative celiac patients. We could not find any statistically significance between EMA-negative celiac patients and controls for the plasma glutamine (928 vs 870 μmol/L) and cystine (31.5 vs 48.5 μmol/L) (p＞0.05). Serum EMA was negatively correlated with plasma cystine (r=-0,321, p=0.023), glutamine (r=-0.413, p=0.003). Conclusions: Our study indicated that plasma glutamine and cystine were significantly lower in the celiac children than the controls. Also, these amino acids were negatively correlated with EMA.

並列關鍵字

cystine ； glutamine ； celiac disease ； endomysial antibody