絲膿桿菌之研究小白鼠接種綠膿桿菌疫苗所産生之保護作用

Studies on Psedomonas Aeruginosa Protection Induced in Mice by Immunization with Pseuclomonas Vacuine

除豐生(Feng-Sheng Chen) ； 戴佛香(Fu-Hsiang Tai)

《中華民國公共衛生學會雜誌》 5期 (1985/09) Pp. 24-37

https://doi.org/10.6288/JNPHARC1985-05-01-02

摘要

本實驗利用小白鼠實驗性膿桿菌致死性傅染之保護作用以評估二膿桿菌菌疫苗。選择當地最常見，毒力高，且對多教抗生素，包括亞米辛(amikacin)珍他徽素(gentamicin)，特卡西林(ticarcillin)，西福他辛(cefotaxi-me)及多豁样菌素B(polymyxin B)有抗果性菌株，Nll24，製出加熱毅死及福雨馬林處理疫苗，每種疫苗均可刺激小白鼠度生高效價膿桿菌菌凝集素；經追加注射後，其效價更高。将此疫苗接種小白鼠，經一定期間，對同型菌株所産生之致死性傅染有濒著之保镬作用。其中接種福雨馬林處理疫苗之小白鼠産生之凝集素效價比加熱毅死疫苗高，但對於同型菌株産生致死性傅染之保镬作用似無明顯之差異。

並列摘要

Two pseudonxnas vaccines were evaluated for their capacity to provide protection from experimental pseudomonas fatal infection in mice. The strain of pseudomonas aeruginosa, N1124, with virulence isolated locally, which is fully resistant to most antibiotics, such as amikacin, gentami - cm, ticarcillin, cefotaxime and polymyxin B, was selected for preparation of vaccine. Two vaccines: the heat killed and formalin treated, each produced high titer of pseudomonas agglutinin in mice by immunization, and higher titer of agglutinin was produced after booster injection. Significant protection against the same pseudomonas strain challenged was conferred by vaccination with these products. Of the two vaccines, higher titer of agglutinin was induced by formalin treated vaccine than the heat killed vaccine. But protection effect seemed to be no significant difference. Since the immunity of pseudomonas aeruginosa is rather type specific, it is required to use polyvalent vaccine for general preventive purpose.