- 期刊
Shen-Yuan-Dan Capsule Inhibiting Inflammatory Reaction by Regulating Insulin Receptor Substrate 1/PI3K/Akt/NF-kB Signaling Pathway in Apoliprotein E Knockout Mice Fed with a High-Fat Diet
摘要
Background: Shen-Yuan-Dan Capsule (SYDC), a traditional Chinese medicine, is proposed to have the capacity to prevent angina pectoris. However, the effects and the related mechanisms of SYDC on atherosclerosis (AS) are still unknown. This study was designed to investigate the effects of SYDC on AS and inflammatory reaction in the apoliprotein E-knockout (ApoE^(-/-)) mice fed with a high-fat diet. Methods: Thirty eight-week-old male ApoE^(-/-) mice were randomly divided into three groups (n = 10) 6 weeks after being fed with a high-fat diet: the control group, the lipitor group, and the SYDC group. The hearts were collected for hematoxylin and eosin (HE) or Van Gieson (VG) staining, and the aortas were collected for quantitative reverse transcription polymerase chain reaction (RT-PCR) and western-blotting. Results: The data showed that the levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), atherosclerotic indexes (AI) and the corrected areas of atherosclerotic plaque of the mice on SYDC group were significantly decreased compared with those of the mice in the control group (p ＜ 0.01, p ＜ 0.05). SYDC can significantly increase collagen proportion in plaques as compared to the untreated mice (p ＜ 0.01). In addition, the messenger ribonucleic acid (mRNA) expressions of insulin receptor substrate 1 (IRS-1), PI3K, Akt, NF-kB and tumor necrosis factor-α (TNF-α) in the mice fed with a high-fat diet were significantly reduced by SYDC (p ＜ 0.05, p ＜ 0.01). Conclusions: SYDC can exert an anti-atherosclerotic effect on ApoE^(-/-) mice fed with a high-fat diet. The action mechanism of SYDC was attributed to its ability to inhibit inflammatory reaction by regulating IRS-1/PI3K/Akt/ NF-kB signaling pathway.
延伸閱讀
- Wilson, J. L., Duan, R., Marakby, A. E., Alhashim, A., & Lee, D. L. (2012). Peroxisome Proliferator Activated Receptor-α Agonist Slows the Progression of Hypertension, Attenuates Plasma Interleukin-6 Levels and Renal Inflammatory Markers in Angiotensin II Infused Mice. PPAR Research, 2012(), 593-599. https://doi.org/10.1155/2012/645969
- Zhang, Y. X., Xu, J. T., You, X. C., Wang, C., Zhou, K. W., Li, P., Sun, P., Wang, L., & Wang, T. H. (2013). Inhibitory Effects of Hydrogen on Proliferation and Migration of Vascular Smooth Muscle Cells via Down-Regulation of Mitogen/Activated Protein Kinase and Ezrin/Radixin/Moesin Signaling Pathways. The Chinese Journal of Physiology, (), 1-10. https://doi.org/10.4077/CJP.2016.BAE365
- Zhang, Y. X., Xu, J. T., You, X. C., Wang, C., Zhou, K. W., Li, P., Sun, P., Wang, L., & Wang, T. H. (2016). Inhibitory Effects of Hydrogen on Proliferation and Migration of Vascular Smooth Muscle Cells via Down-Regulation of Mitogen/Activated Protein Kinase and Ezrin/Radixin/Moesin Signaling Pathways. The Chinese Journal of Physiology, 59(1), 46-55. https://doi.org/10.4077/CJP.2016.BAE365
- Chen, P. H. (2009). Part A. Decoy receptor 3 expression in AsPC-1 human pancreatic adenocarcinoma cells via the phosphatidylinositol 3-kinase-, Akt-, and NF-kappa B-dependent pathwayPart B. Denbinobin inhibits breast cancer migration and metastasis via Src inactivation [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2009.00108
- 廖德翰（2012）。The effects of ankaflavin treatment on insulin signaling and lipid accumulation in high-fat diet mice〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2012.10512