以靜電紡絲法製備含玻尿酸／百里香精油微膠囊成分之蠶絲蛋白奈米纖維膜吸水擴散及抗菌性研究

Preparation of Water Absorption and Antibacterial Nanofiber Membrances of Silk Fibroin/Hyaluronic Acid/Microcapsules of Esential Oils of Thyme by Electrospinning Method

王薇婷(W. T. Wang) ； 徐語彤(Y. T. Hsu) ； 王權泉(C. C. Wang) ； 陳妤(Y. Chen)

《紡織綜合研究期刊》 26卷2期 (2016/04) Pp. 11-21

https://doi.org/10.6439/TTRJ.201604_26(2).0002

摘要

以紡織品做為生醫材料至今日益普及，人們對醫療用品的品質及功能性的需求逐漸提升，且具有特定功能的醫用紡織品的市場需求也不斷地增加。尤其有關具有天然環保及高機能之特用紡織品正廣泛被開發。百里香精油(Essential oils)由體外研究已證實具有良好抗菌性及抗發炎效果，但屬油性材料，無法應用於親水纖維製作且易揮發。因此本研究主要是將精油先用海藻酸鈉包覆製成微膠囊，再以不同比例之玻尿酸、蠶絲蛋白及百里香精油微膠囊經靜電紡絲法製備具抗菌功能之奈米纖維薄膜，並探討纖維膜的抗菌性和吸水膨潤度與擴散係數，且利用SEM觀察纖維的直徑與成絲性。由實驗結果得知，百里香精油微膠囊則對纖維膜抗菌性及纖維細度有影響，當百里香精油微膠囊添加越多時紡絲液黏度增加且纖維細度越大。另外，以定量法進行抗菌測試結果可知，隨著具抗菌性百里香精油微膠囊用量增加其滅菌率也隨之上升，當百里香精油微膠囊添加到30%時，抗菌效果可達97%以上；而玻尿酸對吸水性影響甚大，添加的比例越多，纖維膜的吸水性越好，最高可達到184%；此奈米纖維膜可有效應用於生醫敷材之製作。

關鍵字

蠶絲蛋白 ； 玻尿酸 ； 百里香精油 ； 靜電紡絲 ； 抗菌性

並列摘要

The textile industries used to the medical and related healthcare and hygiene sector are an important and growing trend. The development of textile technology is accelerated, in recent years, the friendly environmental and high functional medical materials have been widely used in related products. Above all, the medical textiles made by electrospinning method had applied to many special fields. In vitro researches have demonstrated very high anti-inflammatory and anti-bacterial activity of essential oils(EOs) of thyme , but that belong to volatile oil-based material. It is difficulty to mix with hydrophilic polymer as spinning solution. The study was aimed at producing microcapsules of thyme EOs and mixed with hyaluronic acid and silk fibroin at various ratio as spinning solution, then made into nanofiber membranes by electrospinning method. The fineness of fibers, absorption and antibacterial properties of membranes were investigated. From the results showed the fineness were increased with amount of microcapsules of thyme EOs. In addition, the antibacterial properties were obviously enhanced by adding microcapsules of thyme EOs. When the amount of microcapsules of thyme EOs was at 30%, the antimicrobial ratio measure up 97.33%. The water absorption of multiple nano-fiber membranes of silk fibroin/hyaluronic acid/ microcapsules of thyme EOs were excellent increased with ratio of hyaluronic acid. The materials have potential to be applicable and available for biomaterials.

並列關鍵字

silk fibroin ； hyaluronic acid ； thyme ； electrospinning ； antibacterial ； microcapsule