Dot blotting顯示九孔的血液細胞有似哺乳動物的Adenosine A1 receptor的存在,而在各種adenosine receptor agonist的測試中發現,adenosine A1 receptor agonist-CHA、R-PIA-會抑制細胞附著,使細胞內cAMP的合成受到抑制、PKA的活性降低,證實九孔血液細胞膜表面確實有具有功能的adenosine receptor存在,且功能跟細胞附著有關。又因non-selective adenosine receptor agonist-NECA、Adenosine-對細胞附著沒有顯著的影響,故推測可能有兩種以上的receptor存在。另一方面,在有caffeine存在的情況下,用adenosine A2A receptor agonist-CGS21680-處理細胞,對細胞附著有加成抑制作用。總結上述結果,九孔血液細胞中應該存在一種與目前已知在哺乳動物細胞不相同的adenosine receptor。
In this study, it was demonstrated that an adenosine A1-like receptor in abalonehemocytes by dot blotting. The adenosine A1 receptor agonists, CHA (N4-cyclo-hexyladenosine) and R-PIA (R-N6-phenyl-isopropyladenosine), showed a strong inhibitory effect on cell adhesion, cAMP synthesis, and PKA activity in abalone hemocytes. Those results pointed out that functional adenosine receptors are indeed exists on abalone hemocytes. However, the non-selective adenosine receptor agonists, NECA (5'-(N-ethylcarboxamido) adenosine) and adenosine have no effect on the hemocyte adhesion. It implied that there might two or more different adenosine receptors. In the presence of caffeine, hemocyte adhesion is synergistically inhibited by CGS21680, an adenosine A2A receptor agonist. Totally, there should have a novel adenosine receptor existing on abalone hemocytes, which is different from that in mammalian cells.