砷暴露地區居民發炎因子基因多形性與頸動脈粥狀硬化之相關性

The Association between Carotid Atherosclerosis and Inflammatory Gene Polymorphisms Among Residents in an Arsenic-Endemic Area

謝宜蓁(Yi-Chen Hsieh) ； 洪啟宗(Chi-Tzong Hong) ； 王淵宏(Yuan-Hung Wang) ； 陳建仁(Chien-Jen Chen) ； 邱弘毅(Hung-Yi Chiou)

《台灣公共衛生雜誌》 24卷1期 (2005/02) Pp. 64-72

https://doi.org/10.6288/TJPH2005-24-01-06

摘要

Objectives: To investigate the association between carotid atherosclerosis and genetic polymorphisms of inflammatory genes among residents in the Lanyan Basin which was a newly confirmed arsenic-endemic area. Methods: Residents aged ≥40 years were recruited and examined for carotid atherosclerosis by Duplex ultrasonography. A total of 332 subjects were randomly selected from the study population as carotid atherosclerosis patients based on their health examination reports with IMT≥1.0 mm and a plaque score ≥1. Controls were 302 subjects with IMT＜l.0 mm and a plaque score＜1. Genetic polymorphisms of IL-1RN, TNF-α, MCP-1, E-selectin, and ICAM-1 were detected by PCR and RFLP. Results: After adjustment for age, sex, tobacco smoking, systolic blood pressure and total cholesterol, the study subjects whose inflammatory gene with variant genetic type have non-significant increased risk for development of carotid atherosclerosis. For study subjects who drank well-water with an arsenic concentration lower than 50μg/L, those who with MCP-1 GIG genotype have a four-fold risk of carotid atherosclerosis than those who with A/A genotype after adjusting the carotid atherosclerosis related risk factors. However, the risk can not be found for study subjects who drank well-water with an arsenic concentration greater than 50μg/L. Conclusions: The study suggested that the arsenic exposure had more significant influence on carotid atherosclerosis than on human susceptibility.

關鍵字

砷 ； 頸動脈粥狀硬化 ； 細胞激素 ； 化學激素 ； 黏附因子

並列摘要

Objectives: To investigate the association between carotid atherosclerosis and genetic polymorphisms of inflammatory genes among residents in the Lanyan Basin which was a newly confirmed arsenic-endemic area. Methods: Residents aged ≥40 years were recruited and examined for carotid atherosclerosis by Duplex ultrasonography. A total of 332 subjects were randomly selected from the study population as carotid atherosclerosis patients based on their health examination reports with IMT≥1.0 mm and a plaque score ≥1. Controls were 302 subjects with IMT＜l.0 mm and a plaque score＜1. Genetic polymorphisms of IL-1RN, TNF-α, MCP-1, E-selectin, and ICAM-1 were detected by PCR and RFLP. Results: After adjustment for age, sex, tobacco smoking, systolic blood pressure and total cholesterol, the study subjects whose inflammatory gene with variant genetic type have non-significant increased risk for development of carotid atherosclerosis. For study subjects who drank well-water with an arsenic concentration lower than 50μg/L, those who with MCP-1 GIG genotype have a four-fold risk of carotid atherosclerosis than those who with A/A genotype after adjusting the carotid atherosclerosis related risk factors. However, the risk can not be found for study subjects who drank well-water with an arsenic concentration greater than 50μg/L. Conclusions: The study suggested that the arsenic exposure had more significant influence on carotid atherosclerosis than on human susceptibility.

並列關鍵字

arsenic ； carotid atherosclerosis ； cytokine ； chemokine ； adhesion molecule

參考文獻

Allen RA,Lee EM,Roberts DH,Park BK,Pirmohamed M(2001).Polymorphisms in the TNF-α and TNF-receptor genes in patients with coronary artery disease.(Eur J Clin Invest).

Allen RA,Lee EM,Roberts DH,Park BK,Pirmohamed M(2001).Polymorphisms in the TNF-α and TNF-receptor genes in patients with coronary artery disease.(Eur J Clin Invest).

Allen RA,Lee EM,Roberts DH,Park BK,Pirmohamed M.(2001).Polymorphisms in the TNF-α and TNF-receptor genes in patients with coronary artery disease.(Eur J Clin Invest).

Barchowsky A,Dudek EJ,Treadwell MD,Wetterhahn KE(1996).Arsenic induces oxidant stress and NF- x B activation in cultured aortic endothelial cells.(Free Radical Biol Med).

Barchowsky A,Dudek EJ,Treadwell MD,Wetterhahn KE(1996).Arsenic induces oxidant stress and NF- x B activation in cultured aortic endothelial cells.(Free Radical Biol Med).