慢性牙周炎組織中NF-κB表現之組織型態分析

牙周炎是一種多因子造成的感染疾病，其發炎反應可能與在牙周組織中調控發炎之轉錄分子NF-κB的表現有關。本研究以臨床上慢性牙周炎患者手術治療時切除之牙周組織，進行牙周組織內轉錄因子NF-κB表現之分析。共收集十四組患者之組織進行石蠟包埋、切片，並以免疫組織化學染色法(immunohistochemistry)進行染色，以便觀察、測量發炎蛋白質NF-κB在慢性牙周炎患者牙周組織中之表現情形。同時以手術區邊緣牙齦組織爲發炎對照組；另收集10位因阻生牙拔除時同步採集之深部牙齦組織作爲健康對照組。由組織形態學(histomorphology)分析得知在病兆區上皮及結締組織中均呈現明顯的NF-κB蛋白質表現現象，此種現象與患者對照組組織之微弱染色結果成強烈對比。此外在健康對照組之NF-κB染色呈現極弱或完全無染色之結果。由本研究得知，臨床上慢性牙周炎患者之患處牙周組織細胞在慢性發炎過程中呈現明顯的NF-κB蛋白質表現。

關鍵字

牙周炎； NF-κB ；慢性發炎；組織型態分析

並列摘要

Periodontitis is a multi-factorial infectious disease that leads to the inflammation and destruction of periodontium. It is possible that this process is associated with the expression of regulatory nuclear transcription factor NF-κB in the inflamed periodontal tissues. The purpose of this study is to evaluate the NF-κB expression in the inflamed granulation tissue in patient with chronic periodontitis, using histomorphological methods. Fourteen patients with chronic periodontitis were included in the study. Both inflamed tissue and healthy tissue were obtained during periodontal surgery. In addition, 10 healthy control samples were obtained from the deep gingival tissue at the extraction sites of impacted teeth in the healthy individuals. All samples were processed with wax embedding, sectioning and immunohistochemical staining. The expression of NF-κB in the gingival tissues was observed and measured under microscope. The results showed that the expression of NF-κB in the inflamed tissues was much more intense than that in the healthy sites in patients with chronic periodontitis. Furthermore, there is no or very scant expression of NF-κB in the control tissues from healthy individuals. In conclusion, the results of this study demonstrated that the expression of NF-κB is associated with the inflammation of periodontal tissues in the patients with chronic periodontitis.

並列關鍵字

Periodontitis ； NF-κB ； chronic inflammation ； histomorphometry

國際替代計量

慢性牙周炎組織中NF-κB表現之組織型態分析

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