Exon 3 of p53 gene is the hot spot region for Oral Squamous Cell Carcinoma

Fatimah Suriati Sulaiman ； Jamil Ahsan Kazi ； Khor Goot Heah ； Rosnah Binti Zain

《Journal of International Dental and Medical Research》 11卷2期 (2018/07) Pp. 398-402

摘要

p53 gene mutations observed at about 50 to 60 % in oral squamous cell carcinoma (OSCC). Until now, most of the studies were focused on exon 5 to 8 of p53 gene instead of the whole exons and it becomes a trend for p53 gene mutation study. However, the site of p53 gene mutation is still in controversy as available techniques showed different results. More importantly, the reports with the same technique demonstrated inconsistence mutation sites. The objective of the present study is to examine the status of p53 gene mutation in all exons (1 to 11). Recently, Multiplex Ligationdependent Probe Amplification (MLPA) technique has proven to be reliable and efficient for the detection of mutation. Therefore, present study used Multiplex Ligation-dependent Probe Amplification (MLPA) to examine p53 gene mutation from exon 1 to 11. DNA specimens from 58 OSCC patients and 10 healthy peoples (controls) were used in this study. Our results demonstrated that 31% (n=58/18) of OSCC patient have p53 gene mutation. Among them 56% (n=10/18) of mutation occurred in exon 3, followed by exon 4 which was 50% (n=9/18). Our study strongly indicated that exon 3 and 4 could be reliable and positive markers of OSCC development and progression. To our knowledge, this study for the first time showed that exon 3 is the hot spot region for p53 gene mutation in OSCC.

關鍵字

Oral squamous cell carcinoma ； p53 gene ； mutations ； exon ； MLPA.