PLGA-Collagen with Citrus paradise extract as Antibacterial Absorbable Surgical Suture

Fadila Nashiri Khoirun Nisak ； Prihartini Widiyanti ； Dwi Gustiono ； Jan Setiawan

《Journal of International Dental and Medical Research》 11卷2期 (2018/07) Pp. 740-744

摘要

Indonesian Central Bureau of Statistics in 2013 is reported that there were 100.106 cases of people getting injured because of accidents. The healing process of the injuries require surgical sutures to unify the wound. Suture either absorbable or non absorbable has possibility to be contaminated by pathogenic microorganism.Thereby, this condition could inhibit tissue healing which might lead to morbidity.This study aim is to make suture which absorb easily and has the antibacterial potency. The suture was made from PLGA - collagen and Citrus paradisi extract. The multifilament fibers of suture was made fromby using electrospinning method. The results of tensile tests showed that the five samples were in the optimal range, 4.62 MPa. The swelling test showed the percentage was less than 20% indicating that no swelling occurred in the wound area. The cytotoxicity assay using MTT method showed the percentage of the living cells reaching up to 90% in all samples.From the degradation test, the optimal value is showed the degradation rate of 89% at 60 days of absorption period. The antibacterial test results using Staphylococcus aureus are obtained the inhibition zone in range of 0,225-1,895 mm indicating that sample had inhibited the bacterial growth. Based on the characterization, PLGA collagen Citrus paradisi extract has potency as antibacterial absorbable surgical suture.

關鍵字

Surgicalsuture ； Absorbable ； PLGA ； Citrus paradise extract