- 期刊
Calcium Transport Protein and Amelogenin Expression in Ameloblastoma: Relationship to Normal Amelogenesis
造釉細胞瘤的運鈣蛋白質與牙釉質形成素之表現：與正常牙釉質形成的關係
摘要
正常牙釉質形成的過程包含表皮造釉母細胞逐漸成熟分化及牙釉基質的形成與基質之鈣化。此過程有一部分是受牙釉質形成素(amologenin)的調節。造釉母細胞的牙釉質形成素蛋白質與運鈣蛋白質的表現與基質形成及鈣化大體上是一致的。齒源性腫瘤與正常牙胚在某些構造與部位在形態上或誘發關係上有某種程度類似的發育過程。在正常牙釉質形成過程中，牙釉質形成素，漿膜鈣離子幫浦(PMCA)，鈣結合素(calbindin)在造釉母細胞的分泌前期階段只有微量的表現。但在牙釉質基質形成與開始鈣化時，才逐漸明顯增加。然而，造釉細胞瘤並不會形成基質或有鈣化現象。為了進一步瞭解造釉細胞瘤於牙釉基質的形成及鈣化過程的調節機制與正常牙胚的差異，本研究利用免疫組織化學法，在十個造釉細胞瘤的病例，發現在柵狀排列的周邊類造釉細胞中都有牙釉質形成素、漿膜鈣離子幫浦及鈣結合素的表現。利用von Kossa切片染色，我們亦發現所有造釉細胞瘤都有鈣離子散佈的現象。因此我們的研究指出造釉細胞瘤的分化階段雖然類似但是卻不同於分泌前期階段的造釉母細胞。我們的研究顯示造釉細胞瘤之細胞外基質形成和鈣化的現象有異常的脫節現象。
並列摘要
The progressive maturation of the ameloblast epithelium, formation of enamel matrix, and the mineralization of that matrix characterize normal amelogenesis. This process is regulated, in part, by the amelogenins. The expression by ameloblasts of amelogenin proteins as well as calcium transport proteins parallels matrix formation and mineralization. Odontogenic tumors reproduce, to varying degrees, the morphological and inductive relationships found among various parts of normal tooth germ. In normal amelogenesis, amelogenin, plasma membrane Ca(superscript ++)-pump PMCA , and calbindin-28KDa calbindin proteins are minimally expressed in presecretory ameloblasts, but progressively increase in expression later as the enamel matrix forms and begins to mineralize. Ameloblastomas, however, do not form matrix or mineralize. In the present study, we demonstrate by immunohistochemistry, that amelogenin, PMCA and calbindin proteins are all expressed in the ameloblast-like palisading peripheral epithelial cells of ten cases of ameloblastoma. In serial sections processed by the von Kossa technique, we also found diffuse calcium in all tumors studied. Our study suggests that ameloblastomas recapitulate a stage of differentiation beyond the presecretory stage and that the mechanisms of extracellular matrix formation and mineralization associated with normal amelogenesis may be uncoupled in ameloblastomas.
延伸閱讀
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