透過您的圖書館登入
IP:52.91.54.203
  • 期刊

Induction of Tomato Jasmonate-Resistant 1-Like 1 Gene Expression Can Delay the Colonization of Ralstonia Solanacearum in Transgenic Tomato

轉殖番茄中 Jasmonate-Resistant 1-like 1 (SlJRL1) 基因的誘導表現能夠延遲番茄青枯菌的菌落

摘要


番茄青枯病是一種非常普遍和廣泛的疾病,主要是由青枯菌引起,特別是在炎熱和潮濕的環境下更為嚴重;於自然環境中,因為寄主抗性的不穩定,以及青枯菌種類與變異性極大,至目前為止,並無良好的防治方式;因此,番茄抵禦青枯菌的分子機制的瞭解仍然有限。本篇報導由番茄釣取到番茄SlJAR1 同源性基因- SlJRL1,並且建立可誘導SlJRL1 基因表現的番茄轉殖株。比對兩基因之蛋白質序列竟然高達99 % 相同性;先前發現SlJAR1 於地下根部之基因表現量最高,相反地,SlJRL1 於地下部表現量最低,反而是地上部有較高的基因表現量;進一步利用南方氏默點法分析,發現於CL5915 番茄品系之基因體含有多個SlJRL1 基因。進一步建構誘導啟動子驅動SlJRL1 之載體,將其轉殖進番茄基因體中,利用DEX 處理轉殖株,其誘導SlJRL1 表現量於第二天即達到高峰;並利用此誘導方式,進行青枯菌感染,經DEX 誘導之轉殖番茄可延緩萎凋病徵發生;並分析體內之青枯菌族群,發現轉殖番茄經由DEX 誘導後,亦可減緩於莖頂處青枯菌累積之數量;由此可知,植物茉莉酸荷爾蒙具有參與番茄防禦青枯菌感染之功能。因此,本篇SlJRL1 轉殖番茄之結果,可提供抵抗番茄青枯病之分子機制的瞭解。

並列摘要


The bacterial wilt in tomato caused by Ralstonia solanacearum infection is common and widespread, especially in hot and humid environments. Combating this disease is difficult due to unstable host resistance and the variation and diversity of the bacterial strains. Thus, the molecular mechanisms underlying tomato resistance against Ralstonia solanacearum remain unknown. Here, we isolated a homolog of tomato Solanum lycopersicum Jasmonate-Resistant 1 (SlJAR1), named SIJAR1-like 1 (SlJRL1), and generated transgenic tomato lines harboring an inducible promoter-driven SlJRL1 construct. SlJRL1 shares 99% amino acid identity with SlJAR1. Intriguingly, SlJRL1 showed preferential expression in aerial parts and SlJAR1 in roots. DNA gel blot analysis revealed multiple copies of SlJRL1 in the tomato genome. Transgenic tomato containing a single copy of the transgene SlJRL1 exhibited high levels of SlJRL1 expression two days after dexamethasone (DEX) induction. Moreover, DEX-induced SlJRL1 expression could delay the symptoms of tomato bacterial wilt, and efficiently reduce the amount of Ralstonia in stems. The phytohormone jasmonic acid may play a role in this resistance response. This study of inducible SlJRL1 expression in transgenic tomato contributes to the molecular understanding of tomato resistance against bacterial wilt.

延伸閱讀