Recently, fluorescent probes have become an important tool in medical diagnosis because they can rapidly and sensitively detect target analytes. However, quantitative detection of trace amounts of a biomarker in protein rich samples using fluorescent probes is a great challenge as the real signal is usually obscured by nonspecific fluorescence. We have designed several novel fluorescent probes that can overcome the problems of nonspecific fluorescence and applied them in no-wash live cells imaging. Currently, we are improving the fluorescent probes performance. We believe that they can be applied in biomedical analysis and environmental pollutants monitoring in the near future.
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