多價的表面修飾與醣奈米材料的生醫應用

Multivalent Surface Modification and Glyco-nanomaterials in Biomedical Applications

賴千蕙(Chian-Hui Lai)

《化學》 75卷3期 (2017/09) Pp. 241-258

https://doi.org/10.6623/chem.2017027

摘要

本篇回顧性的文章，指在介紹修飾奈米尺度的材料與利用多價作用（Multvalent Effect）在各種生醫方面的應用，尤其著重於利用不同醣體表面修飾的奈米載體，包含發展多功能性奈米粒子、藥物標把輸送、測量醣體－醣體的極微小作用力、腦部中風後MRI的顯影劑、細菌偵測探針等。另外，介紹兩種生物晶片，包含蛋白質微陣列晶片和微流體生物晶片的表面蛋白質或是抗體專一位向固化。硼酸生物晶片可專一性固化含有Fc特殊結構區域（Fc domain）之凝集素蛋白質（Lectin），增強檢測靈敏度；微流體生物晶片方面，以化學方式修飾抗體Fc結構區域，使抗體位向專一固化於微流體生物晶片，因此抗體活性區域Fab結構區域的完全表露，增強檢測訊號與抓取血液中稀少的循環癌細胞（rare circulating tumor cells）。將針對各種化學製程方法與其鑑定做介紹。

關鍵字

奈米尺度 ； 多價作用 ； 醣奈米粒子 ； 生物探針 ； 藥物輸送 ； 生物晶片 ； 位向專一的抗體

並列摘要

The reviewer article introduces several nano-scale materials and using their unique corresponding multivalenct effect property to develop different bio-applications. The article specially focus on different glyco-modified nano-scale carriers' bioapplications, including development of multi-functional nanoparticle, designe targeting drug-delivery, measurement of carbohydrate-carbohydrate ultra-weak interaction, the MRI agent for stroke and the bacterial detection probes. Besides, two types of bio-chips including glass protein microarray and microfluidic chip are introduced for site-specific protein or antibody immobilization, respectively. Boronic acid microarray surface can site-specifically immobilize Fc-fused protein (Lectin-Fc chimara) on it and therefore the lectin microarray enhances its sensitivity. The channel inner surface of microfluidic chip is immobilized antibody via site-specific Fc domain position, and therefore the active Fab site of antibody for antigen recognition on cell membrane is fully exposed. The microfluidic chip was applied on capture rare circulating tumor cells and was shown better capture ability. The review would describe different chemical modification methods on diverse nano-materials' surface and their characterizations.

並列關鍵字

Nano-scale ； Multivalent effect ； Glyco-nanoparticle ； Bioprobe ； Drug-delivery ； Biochip ； Site-specific antibody