本研究提出使用羧甲基纖維素(CMC)進行纖維素試紙平臺改質。改質後的平臺具備高靈敏性與穩定度,可用於快速、精確檢測結核菌素蛋白衍生物(Tuberculin Purified Protein Derivative, PPD)。將合成的羧甲基纖維素吸附於紙張基材上,可在親水環境下固定生物分子,提升紙張平臺功能性與穩定性。此外,生物識別元素可以透過羧甲基纖維素修飾纖維素材料也可以與EDC/NHS交聯,以產生能夠與配體或目標分析物共價鍵結NHS酯基(NHS ester)。利用改質後的系統檢測結核菌素蛋白衍生物不僅能夠達到最小偵測極限0.3 ng/mL,多個樣本亦可在30分鐘內檢測完成。這些結果表明本篇研究提出的表面改質分析裝置(surface modified paper-based analytical device, sPAD),在資源有限環境下,具有高靈敏度和高通量的現場疾病診斷能力。
In this study, the sensitive and stable cellulose paper platform modified with carboxymethyl cellulose (CMC) was investigated for use as a rapid and accurate immunoassay for tuberculosis. Absorbing CMC to the paper substrate enables it to act as a linker for immobilizing biomolecules in a hydrophilic environment to promote its functionality and stability. This CMC-modified cellulose material can also be cross-linked with 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride/N-Hydroxysuccinimide (EDC/NHS) to produce NHS ester groups that can be covalently conjugated with ligands or target analytes. As a result, we were able to achieve a detection limit of 0.3 ng/mL for tuberculin purified protein derivative. Besides, the turnaround time for multiple tests can be completed in as little as 30 min. These results suggest that the proposed surface-modified paper analytical device (sPAD) possesses the capability for sensitive and high-throughput on-site disease diagnosis in resource-constrained settings.
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