The purpose of this study was to examine muscle function, anaerobic power capacity and aerobic capacity depending on α-actinin-3 (ACTN-3) gene polymorphism of speed roller skaters. RR, RX, and XX types were determined using polymerase chain reaction (PCR) in 29 male and 26 female elite players. Isokinetic muscular function of the knee joint for sprint and long-distance events was measured by the peak torque and the total work through left and right flexors and extensors, respectively, at 60°/s for 5 repetitions and 180°/s for 26 repetitions. Anaerobic power capacity, peak power, mean power, and fatigue index were analyzed using the Wingate measurement method over 30 seconds. Aerobic capacity, maximum oxygen consumption (VO_(2max)), maximum heart rate (HR_(peak)), and anaerobic threshold (AT) level were analyzed by using Bruce protocol of maximal treadmill test. We found that sprinters in speed roller skating have a higher RR and RX frequency, whereas long-distance skaters have a higher XX frequency. In addition, RR skaters showed better anaerobic capacity than XX skaters, whereas XX skaters showed better aerobic capacity than RR skaters. Among all ACTN-3 gene polymorphisms, RR genotype is advantageous to sport events in which anaerobic power capacity works as a determinant of performance, while XX genotype is advantageous to sport events in which aerobic capacity is required to improve performance. Therefore, these two types of ACTN-3 gene polymorphism maybe appropriate for forecasting speed roller skaters' aerobic and anaerobic capacity of sport performance.