接種污泥對連續批式反應器好氧生物顆粒化之影響

Effects of inoculated sludge on aerobic granulation in sequencing batch reactor

林彬(Pin Lin)

指導教授 ： 李篤中

國立臺灣大學/工學院/化學工程學研究所/碩士(2009年)

https://doi.org/10.6342/NTU.2009.00423

摘要

本論文探討移除活性污泥中胞外高分子物質及接種不同來源之污泥對於好氧生物顆粒化之影響。
首先分別以原始活性污泥及移除胞外高分子物質後之污泥進行接種，並利用葡萄糖作為基質碳源於連續批式反應器中培養好氧顆粒。活性污泥在移除胞外高分子物質後其好氧顆粒形成時間約為3週，原始污泥之顆粒形成時間約為2週。原始污泥所形成之顆粒有較多真菌。利用變性梯度電泳檢視兩系統之真菌相，Pseudoplatyophrya nana及Candida sp. BG02-6-6-1-7兩菌種出現在原始污泥之顆粒化初期，但並未出現在移除過胞外高分子物質之污泥其顆粒化過程中，其可能為原始污泥顆粒化較快發生的原因。兩系統之顆粒最後皆崩解，而Opisthonecta minima及Candida solani兩真菌種及Sphaerotilus sp.之細菌種皆在此時出現。
而在以乙酸進料的條件下，原始污泥及移除胞外高分子物質後之污泥其顆粒化過程則無明顯差別。以葡萄糖所培養之好氧顆粒其菌屬組成包含有：Paracoccus屬、Acidovorax屬、Pseudomonas屬等。而以乙酸所培養之好氧顆粒其菌屬組成包含有：Stenotrophomonas屬、Brevundimonas屬等。

關鍵字

好氧生物顆粒 ； 連續批式反應器 ； 變性梯度膠體電泳

並列摘要

Effects of removal of extracellular polymeric substances (EPS) in activated sludges on aerobic granulation process and aerobic granulation taken sluges from two wastewater treatment plants as seeds were studied.
Glucose was utilized to cultivate granules in two sequencing batch reactors (SBRs) that were seeded with original sludge (S-s) or EPS-free sludge (S-p). The S-s sludge granulated in two weeks, faster than the S-p sludge did (three weeks). The fungi were predominated in the S-s system. The denaturing gradient gel electrophoresis (DGGE) results determined that Pseudoplatyophrya nana and Candida sp. BG02-6-6-1-7 appeared in the starting phase of granulation period in S-s but not in S-p sludge, probably accounting for the earlier granulation for the former system. The fungus like Opisthonecta minima and Candida solani and bacterial species like Sphaerotilus sp. appeared in the granule disintegration phase.
Acetate was utilized to cultivate granules in SBRs seeded with sludge and EPS-extracted sludge. The two seeds yielded similar granulation processes. Glucose-fed granules were composed of genus like Paracoccus、Acidovorax、Pseudomonas while acetate-fed granules were composed of Stenotrophomonas、Brevundimonas.

並列關鍵字

aerobic granule ； SBR ； DGGE

參考文獻

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