仿生明膠塗層含鎂矽酸鈣陶瓷多孔支架應用於骨組織工程

Biomimetic Gelatin Coating Magnesium-Contained Calcium Silicate Ceramic Scaffold for Bone Tissue Engineering

劉育瑋(Yu-Wei Liu)

指導教授 ： 高嘉澤

中山醫學大學/口腔醫學院/口腔科學研究所/碩士(2020年)

https://doi.org/10.6834/CSMU201900280

摘要

在過去的十年中，三維列印（3D Printing）作為一種新興的技術良好地控制生物支架的型態、孔徑尺寸和孔隙率，也因此在組織工程研究方面取得了突破性的重大進展，以實現非常複雜的三維空間組織重建使其成為未來再生醫學的重要潛力股之一，生物陶瓷各項相關研究在其中尤為受到重視，以矽酸鈣（Calcium Silicate , CS）為基礎而發展起來的生物陶瓷材料具有骨誘導性質，本實驗室過去已自行開發出含鎂矽酸鈣生物陶瓷，該材料具有良好的生物降解能力與其釋放之離子能促進多種幹細胞增殖分化的效果證明能加速並提升硬組織的再生，並添加高分子材料以提升其列印性，但是由於高分子的添加使得材料較為疏水不利細胞貼附，本研究透過三維列印技術製備多孔結構陶瓷支架後經由天然具有生物相容性明膠以不同比例作為生物活性塗層，進行機械性質、體外降解與生物活性等相關分析測試，期望能夠改善陶瓷材料親水性與提升各項生物活性指標。實驗結果顯示新開發之含有明膠塗層陶瓷支架在降解性能方面能夠延緩支架降解為骨組織缺損修復提供更多時間外，在生物活性的部分具有良好生物相容性與較高的鹼性磷酸酶活性，於鈣沉積染色實驗也顯示出更好的結果，進一步在動物實驗結果當中顯微電腦斷層掃描中明顯觀察到相較於其他組別有較好的骨再生效果，因此本研究證實由三維列印製備明膠生物塗層含鎂矽酸鈣多孔支架能提供足夠的機械性質外，加上不同材料所釋放的離子提升組織再生能力，在未來能夠做為臨床骨組織修復當中有潛力的生物陶瓷材料。

關鍵字

三維列印 ； 矽酸鈣 ； 鎂 ； 明膠 ； 生物陶瓷 ； 骨組織工程

並列摘要

In the past decade, 3D printing has evolved into an emerging technology that can better control the scaffold shape, pore size, and porosity and apply it to tissue engineering. It has made breakthroughs in tissue engineering research by achieving very complex 3D reconstruction and making it become one of the important potential reserves for future regenerative medicine. Bioceramics related research is especially valued in tissue engineering. Calcium silicate based ceramic materials have unique osteoinductivity and our laboratory has developed magnesium-contained calcium silicate bioceramic. It has been confirmed by experiments that magnesium-contained calcium silicate has good biodegradability and promotes stem cell proliferation and differentiation. However, due to the addition of the polymer for printability, the composite become more hydrophobic to hinder cell attachment. In this study, the biocompatible gelatin was added as a bioactive coating in different proportions after the porous structure ceramic scaffold was prepared by 3D printing technology. It is expected to improve the hydrophilicity of the composite scaffold and promote the biological activity. The mechanical properties, in vitro degradation and cell experiments were also analyzed. The experimental results show that the mechanical properties were enhanced provided more time for bone regeneration in terms of the performance of delayed degradation. The scaffold also showed better biocompatibility and higher alkaline phosphatase activity as well as alizarin red staining. Animal study indicated better results of new bone formation and immunohistochemistry than other groups. Therefore, this study demonstrated that the preparation of gelatin bio coated magnesium-containing calcium silicate ceramic porous scaffolds may enhance bone tissue regeneration and could be used for clinical bone tissue applications in the future.

並列關鍵字

3D printing ； Calcium silicate ； Magnesium ； Gelatin ； Bone tissue engineering

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