- 學位論文
血清中幽門螺旋桿菌抗體之定量檢驗 參考值研究
The Reference Value Study of Quantitative Inspection for Helicobacter pylori antibody of Serum
摘要
目前市面上的幽門螺旋桿菌抗體檢測試劑，主要作為定性檢測，是一種乳膠微粒凝聚之試劑，其原理是將取自幽門螺旋菌之抗原被附在細小之乳膠粒上，當乳膠微粒與血清中幽門螺旋菌之抗體混合時，由於抗原抗體之反應產生凝聚現象，在三到五分鐘內肉眼就可以辨識出是否有受到感染而產生抗體。 本研究中的幽門螺旋桿菌抗體IgG 檢驗試劑專門用於日立全自動生化分析儀，定量檢測人血清中特異的幽門螺旋桿菌抗體IgG，用於輔助診斷幽門螺旋桿菌的感染。本研究報告了幽門螺旋桿菌抗體IgG 檢驗試劑正常參考值的確定方法及步驟。 希望利用全自動生化分析儀以免疫比濁法，定量檢測人血清中幽門螺旋桿菌抗體IgG的濃度，用於輔助診斷常見疾病如慢性胃炎、胃潰瘍、十二指腸潰瘍等，除了為社會大眾提供更快速更精準篩檢幽門螺旋桿菌的輔助工具，也可以取代目前市面上的定性檢測試劑，藉由定量數值檢測提供給臨床醫師更完整的評估數據。
關鍵字
幽門螺旋桿菌並列摘要
The test Reagents of Helicobacter pylori antibody in the current market, mainly as a qualitative detection, is a kind of reagents of Latex particle cohesion, the principle is that access to antigen of helicobacter pylori attach on small Latex particle, When the mixture Latex particle with Helicobacter pylori antibody of Serum, as the Condensation phenomena is generated by reaction of Antigen-antibody, The naked eye to distinguish Antibody production once Infected during 3 to 5 minutes. The study of test Reagents of Helicobacter pylori antibody IgG Specifically for Auto-Biochemical analyzer of Hitachi, quantitative inspection for specific Helicobacter pylori antibody IgG of Serum, for auxiliary to Diagnosis infection of Helicobacter pylori. In this study, report to method and step for determining of the normal reference value of the test Reagents of Helicobacter pylori antibody IgG. Wants to use auto-Biochemical analysis on Immunity- Turbidimetric assay, Concentration quantitative inspection for Helicobacter pylori antibody IgG of Serum, for auxiliary to diagnosis common diseases such as Gastritis, Gastric ulcer, Duodenal ulcer.... In addition to provide more fast and precise screening aids of Helicobacter pylori, also replace the test reagents of qualitative detection in the current market, provide more complete assessment data to Clinicians by Quantitative Inspection.
並列關鍵字
Helicobacter pylori參考文獻
延伸閱讀
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