青黴菌和傘枝犁頭黴之幾丁聚醣分子量的分析

Molecular Weight Analysis of Chitosan Extracted from Penicillium sclerotia and Absidia coerulea

林惠玲(Hui-Ling Lin)

指導教授 ： 許垤棋

大同大學/工程學院/生物工程學系所/碩士(2012年)

摘要

幾丁聚醣是陽離子型、具生物相容性、無毒以及可被生物分解的多醣，可廣泛應用於食品、藥品以及化妝品。幾丁聚醣是某些真菌細胞壁中的重要的組成，本研究從青黴菌(Penicillium sclerotia) BCRC 33160及傘枝犁頭黴(Absidia coerulea )BCRC 32968的菌絲中萃取，以瞭解幾丁聚醣的產量及分子量。分子量的測定以凝膠滲透層析管柱，藉由高效能液相層析儀，以及折射率偵測器完成偵測。青黴菌的幾丁聚醣產量很少，重量平均分子量是28.1 kDa。傘枝犁頭黴的產量是每公升0.65公克，重量平均分子量是30.7 kDa。由結果可知傘枝犁頭黴BCRC 32968的幾丁聚醣的產量顯著優於青黴菌。

關鍵字

真菌幾丁聚醣 ； 分子量

並列摘要

Chitosan is polycationic, biocompatible, nontoxic as well as biodegradble, and has been reported to have numerous applications, especially in food, pharmaceutical and cosmetic industries. Chitosan is a substantial component of the cell wall of certain fungus. In this work, chitosan was extracted from Penicillium sclerotia BCRC 33160 and Absidia coerulea BCRC 32968, which were investigated for their yield and the distribution of molecular weight. Relative molecular weight of fungal chitosan was determined by gel permeation chromatography(GPC) by HPLC on a Shodex SB-804 column with a refractive index detector. Chitoasn yield of Penicillium sclerotia BCRC 33160 was low, and the weight-average molecular weight (Mw) was 28.1 kDa. Chitoasn yield of Absidia coerulea BCRC 32968 was 0.65 g/L, and the weight-average molecular weight (Mw) was 30.7 kDa . The result indicates that Absidia coerulea BCRC 32968 produced much more chitosan than Penicillium sclerotia BCRC 33160.

並列關鍵字

molecular weight distribution ； fungal chitosan

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