雙層多孔性ePTFE膜以電漿鍍膜對細胞活性之評估

本研究的主要是利用氬氣低溫電漿活化拉伸性鐵氟龍(expanded polytetrafluoroethylene，ePTFE)膜的表面，使其表面產生活化自由基後，再利用UV光誘導接枝poly(ethylene glycol) methacrylate (PEGMA)或acrylamid (AAm)，使ePTFE膜表面產生親水性的官能基，如C=O、－CONH2等，期待改善膜表面的親水性。改質後的ePTFE-g- AAm與ePTFE-g- PEGMA膜，以接觸角觀察表面親水性的變化，再分別以化學分析電子光譜儀(ESCA)、傅立葉轉換紅外線光譜儀量測原理(FTIR)及掃描式電子顯微鏡(SEM)觀察改質前後的結構、化學結構變化和表面型態。當ePTFE 表面接枝聚合AAm或poly(ethylene glycol) methacrylate (PEGMA)後，再利用化學交聯法以架橋劑：碳化二亞胺(EDC，5wt%) 即可於表面固定生物相容性物質，如cRRE、collagen，以增加ePTFE 材料的生物相容性，利用人類臍靜脈內皮細胞（Human Umbilical Vein Endothelial Cells；HUVECs）與纖維母細胞(CCD966SK fibroblasts)進行ePTFE改質膜雙面培養細胞之貼附性測試，以人類臍靜脈內皮細胞培養之單面以AAm固定多肽鏈cRRE及AAm固定膠原蛋白collagen的表面對HUVECs的貼附性較佳；而另ㄧ面以纖維母細胞培養之單面於改稙膜上並無較好的貼附率，但在增加其培養天數至ㄧ週，以AAm及PEGMA固定膠原蛋白則有較好的貼附率，不同細胞有不同生長週期，在雙面培養上細胞的培養生長週數是個變因，以確立此改質方法於生醫領域的應用。

關鍵字

拉伸性鐵氟龍膜；電漿鍍膜； UV光誘導接枝；人類臍靜脈內皮細胞；人類纖維母細胞

並列摘要

Expanded polytetrafluoroethylene (ePTFE) films, was treated with argon plasma to improre the hydrophilicity. To create active site on the surface of ePTFE films, the coupling reaction with poly(ethylene glycol) methacrylate (PEGMA) or acrylamid (AAm) was initiated with UV. The contact angle was improved from 135°to 0°for modified ePTFE film with grafting poly(ethylene glycol) methacrylate (PEGMA；10wt%, and 30 minutes). The surface properties of ePTFE-g-PEGMA films and ePTFE-g-AAM films were also characterized by electron spectroscopy for chemical analysis, fourier transform infrared spectroscopy and scanning electron microscope. Peptide cRRE and collagen was crosslinked with (N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide)(EDC 5w%) on ePTFE-g-PEGMA films and ePTFE-g-AAM films. Human umbilical vein endothelial cells on one side of the ePTFE-g-AAm fixed polypeptide chain cRRE and ePTFE-g-AAm fixed collagen of the surface of HUVECs attached to the better,on the other hand the surface of human fibroblasts there is no better attachment rate of change menbrane, increasing the culture days to one weeks, better attached to the ePTFE-g-AAm and ePTFE-g-PEGMA fixed collagen rate in order to establish this modification in the field of biomedical applications. Different cells have different growth cycles, some the cell growth pay attention to develop the number of days in the double-sided .That can be enhance the biocompatibility of the film.

並列關鍵字

ePTFE ； CCD966SK fibroblasts ； Human Umbilical Vein Endothelial Cells

參考文獻

[10] 曾勝群，“利用丙烯酸電漿聚合法對雙軸延伸PTFE表面改質之研究 ”，私立中原大學化學研究所碩士學位論文，2006

[13] 吳昱欣，“血管新生數學模型之探討”，私立大同大學生物工程學系

[12] 廖慧帆，“輕量化高分子微載體對細胞貼附及細胞產物生產之影響”，私立大同大學生物工程學系碩士學位論文，2008

[11] 廖淑娟，“利用電漿沉積及後處理固定耐水親水性薄膜及其濕度感測特性研究”，私立大同大學材料工程學系碩士學位論文，2007

[16] A.P. Zhu, Zhang Mingc, Shen Jian. 2005. “Blood compatibility of chitosan_heparin complex surface modified ePTFE vascular graft”. Surface Science 241：485–492

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雙層多孔性ePTFE膜以電漿鍍膜對細胞活性之評估

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