仿生矽化包覆酯解酶添加溶劑正己烷生產生質柴油

Biodiesel production using lipase encapsulated in biomimetic silica with n-hexane as solvent

李家綺(Chia-Chi Lee)

指導教授 ： 游吉陽

大同大學/工程學院/生物工程學系所/碩士(2012年)

摘要

本研究係利用矽酸(由tetramethoxysilane水解產生)及polyallylamine進行仿生氧化矽包覆lipase (from Pseudomonas cepacia )，並以包覆後酵素催化大豆油或廢食用油與甲醇進行轉酯化反應。利用反應曲面法及Box-Behnken實驗設計分別探討反應溫度(35 - 50℃)、醇油莫耳比(3：1 - 5：1)及溶劑n-hexane添加量(25 - 75%, w/w)對生質柴油轉化率之影響。大豆油最適化條件為43.6℃、醇油莫耳比4.3：1及n-hexane添加油重之75%；預期與實驗轉化率分別為79%及76%。廢食用油最適化條件則為43.3℃、醇油莫耳比5：1及n-hexane添加油重之38%，預期與實驗轉化率分別68%及67%。以大豆油為油源，儲存於4℃或室溫一個月後，生質柴油轉化率均無下降。

關鍵字

正己烷 ； 反應曲面法 ； 生質柴油 ； 酯解酶 ； 仿生氧化矽 ； 助溶劑

並列摘要

Lipase from Pseudomonas cepacia was encapsulated in biomimetic silica which was mediated by polyallylamine. The encapsulated lipase was applied to biodiesel production with soybean oil or waste cooking oil as feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) , and n-hexane concentration (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6℃, substrate molar ratio 4.3：1, and 75% n-hexane. The predicted and experimental values of biodiesel conversion for soybean oil were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3℃, substrate molar ratio 5：1, and 38% n-hexane. The predicted and experimental values of conversion for waste cooking oil were 68% and 67.2 %, respectively. When the encapsulated lipase was stored at 4℃ or room temperature for 1 month, the conversion remained the same with soybean oil as feedstock.

並列關鍵字

response surface methodology (RSM) ； biodiesel ； lipase ； biomimetic silica ； n-hexane ； solvent

參考文獻

蔡秉宏. 2012. 仿生氧化矽包覆酯解酶生產生質柴油之最適化. 生物工程系, 碩士論文, 大同大學.

張雅琪. 2008. 嗜鹽菌Haloferax mediterranei生產類胡蘿蔔素之最適化探討. 生物工程系, 碩士論文, 大同大學.

陳佳偉. 2009. 固定化脂肪分解酵素應用於大豆油水解之研究. 化學工程學系, 碩士論文, 成功大學. 陳國誠. 2000. 生物固定化技術與產業應用. 茂昌圖書有限公司.

Chen, H.C., Ju, H.Y., Wu, T.T., Liu, Y.C., Lee, C.C., Chang, C., Chung,

Betancor, L., Luckarift, H.R. 2008. Bioinspired enzyme encapsulation for biocatalysis. Trends in Biotechnology, 26(10), 566-572.