L-myc和麩胺基硫轉移酶M1之基因多形性與肝細胞癌感受性的相關研究

Polymorphism of l-myc and Glutathione S-Transferase M1 Genes and Susceptibility to Hepatocellular Carcinoma

黃仁彰(Jen-Chang Huang) ； 陳建仁(Chien-Jen Chen) ； 廖運範(Yun-Fan Liaw) ； 于明暉(Ming-Whei Yu) ； 謝玲玲(Ling-Ling Hsieh)

《中華公共衛生雜誌》 15卷5期 (1996/10) Pp. 447-456

https://doi.org/10.6288/CJPH1996-15-05-05

摘要

環境因子和遺傳因子可能皆和肝細胞癌的發展有關。因此本研究嘗試以病例對照研究來探討B型／C型肝炎病毒感染，及宿主L-myc和GST M1基因之多形性與肝細胞癌感受性的相關性。以聚合酶鏈鎖反應分析每個個體的L-myc和GST M1永因型，研究對象包括73位病例和以年齡、性別變項進行頻率配對取樣的191位健康對照。
結果要現GST M1基因型在病例、對照組之間沒有分布上的差異，L-myc基因型在兩組之間則有分布上的差異；和SS基因型比較，屬LS基因型個體罹患肝細胞癌的相對危險性為1.48（95%信賴區間0.69-3.17），屬LL基因型的相對危險性為2.12（95%信賴區間0.96-4.69），以趨勢檢定分析達邊緣性統計意義(P=0.051)。進一步以B型肝炎病毒表面抗原進行分組，姿現在帶原者組別中，屬LL基因型個體罹患肝細胞癌的相對危險性為1.80（95%信賴區間0.67-4.94），屬LL基因型個體的相對危險性為3.05（95%信賴區間1.10-8.70），達統計上顯著差異(P=0.021)。在年齡不大於50歲和男性的組別中，也發現相似的結果。對數複迴歸分析也顯示在調整肝炎病毒標記、GST M1基因型等變項復，L-myc LL基因型和肝細胞癌之間仍有顯著相關的情形。綜合言之，雖然目前對於不同L-myc基因型所產生不同的癌變機率，其分子機轉尚未明瞭，不過本研究結果顯示帶有L-myc SS基因型的個體可能有較低的相對危險性罹患肝細胞癌。

關鍵字

L-myc前致癌基因 ； GST M1基因 ； 肝細胞癌

並列摘要

Both environmental and genetic factors may be involved in hepatocarcinogenesis. This study was carried out to investigate the relationship between the host L-myc and GST M1 genetic polymorphism and susceptibility of HCC. Using a polymerase chain reaction (PCR)-based assay on DNA extracted from liver and peripheral blood samples, the L-myc and GST M1 genotype of 73 HCC cases and 191 age-sex matched healthy controls was determined. This case-control study showed a slight difference in the distribution of L-myc genotypes between HCC patients and controls (p=0.l24). Compared with individuals who carried the SS genotype, the odds ratio associated with HCC was 1.48 (95% confidence interval [CI]=0.69-3.17) for those who carried the LS genotype and 2.12 (95%CI=0.96-4.69) for those who carried the LL genotype, respectively. There was a significant trend for the odds ratio of developing HCC with an increasing number of the L allele among men, hepatitis B virus carriers, and individuals aged 50 years or younger. Multiple logistic regression analysis indicated that persons with L-myc LL genotype tend to have a higher risk to develop HCC. There was no difference in the distribution of GST M1 genotypes between HCC patients and controls (p=0.499).

並列關鍵字

L-myc proto-oncogene ； GST M1 gene ； hepatocellular carcinoma