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Efficacy of "Sargassum oligocystum" Extract on the Innate Immunity of White Shrimp "Litopenaeus vannamei" and Its Resistance Against "Vibrio alginolyticus"

馬尾藻"Sargassum oligocystum"萃取物影響白蝦先天性免疫以及對溶藻弧菌抵抗效能

摘要


本實驗研究白蝦接受馬尾藻"Sargassum oligocystum"萃取物(SOE)後之免疫反應,以及對溶藻弧菌"Vibrio alginolyticus"之抵抗。蝦浸泡於含不同SOE濃度(0、100、300及500 mg/l)海水,經0.5、1、3及4小時,發現血淋巴球數、原酚氧化酵素活性、呼吸爆、超氧離子歧化酵素活性、麩胱甘月太 過氧化酵素活性、以及溶菌酉每 酵素活性隨著SOE濃度增加而增加。白蝦浸泡於含SOE海水,經過4小時,脂多糖與葡聚糖結合蛋白(LGBP)與黏附蛋白(peroxinectin)基因表現增加。蝦浸泡於含SOE 100, 300及500 mg/l海水4小時後,發現造血組織血淋巴球增生。蝦浸泡含SOE 100, 300及500 mg/l海水,對溶藻弧菌的吞噬能力及清除效率增加。浸泡於含SOE 100, 300及500 mg/l海水,經過4小時,再接受溶藻弧菌攻擊(4.5×10^(6) cfu/shrimp),發現浸泡於300及500 mg/l SOE海水的白蝦,再接受弧菌後12~120小時後,存活率較高。本研究證實白蝦浸泡於含300 mg/l SOE海水,其血淋巴球數與免疫因子增加、LGBP與PX基因表現增加,造血組織血淋巴球增生,對溶藻弧菌抵抗,吞噬能力及清除效率增加。

並列摘要


This study was to examine immune response of white shrimp "Litopenaeus vannamei" and its resistance against "Vibrio alginolyticus" when shrimp received "Sargassum oligocystum" extract (SOE). Shrimp which had been immersed in seawater containing SOE at 300 and 500 mg/l for 4 h, hemocyte count and other immune parameters including phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, and lysozyme activity significantly compared to the control shrimp. The expressions of LGBP and PX, and mitotic index of hematopoietic tissue (HPT) of shrimp immersed in SOE at 100, 300 and 500 mg/l after 4 h were significantly higher than those of control shrimp. Shrimp immersed in 100, 300, and 500 mg/L SOE showed increased phagocytic activity and clearance efficiency to "V. alginolyticus". Shrimp which had been immersed in seawater containing 100, 300 and 500 mg/l SOE after 4 h were challenged with "V. alginolyticus" at 4.5 x 10^(6) colony-forming unit (cfu)/ shrimp, and then placed in seawater. The survival rate of shrimp immersed in 300 and 500 mg/l SOE was significantly higher than that of control shrimp over 12~120 h. In conclusion, shrimp immersed in seawater containing SOE at 300 mg/l increase resistance, phagocytic activity and clearance efficiency against "V. alginolyticus" as an evidence of increases in hemocyte count, other immune parameters, expressions of LGBP and PX, and mitotic index of HPT.

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