Endemic fluorosis in Henan province, China: ERα gene polymorphisms and reproductive hormones among women

中国河南省氟病区女性ERα基因多态性与生殖激素的关系

Guo-Yu Zhou ； Li-Jun Ren ； Jia-Xiang Hou ； Liu-Xin Cui ； Zhong Ding ； Xue-Min Cheng ； Jing-Yuan Zhu ； Rui-Rui Cui ； Yue Ba

《Asia Pacific Journal of Clinical Nutrition》 25卷4期 (2016/12) Pp. 911-919

https://doi.org/10.6133/apjcn.062016.01

摘要

背景与目的：探讨氟暴露及ERα基因多态性对氟病区女性生殖激素浓度的影响。方法与研究设计：按照饮用水中氟浓度分为氟病区组、改水组和对照组。采用整群抽样的方法选择中国通许县居住的679名成年女性为研究对象，采集研究对象空腹静脉血及晨尿样本，并测量血清促性腺激素释放激素（gonadotropin releasing hormone, GnRH）、卵泡刺激素、促黄体生成素、雌二醇和尿氟浓度。结果：改水组携带ERα rs3798577C/C基因型女性血清GnRH浓度低于携带C/T和T/T基因型女性（p＜0.05）。氟病区组携带ERα PvuII Pp基因型女性血清GnRH浓度低于携带PP和pp基因型女性（p＜0.05）。携带ERα PvuII Pp、pp和ERα XbaI Xx、xx基因型的女性血清GnRH浓度低于对照区携带相同基因型的女性（p＜0.05）。此外，无论其携带的ERα rs3798577基因型是C/C、C/T或T/T，氟病区组女性血清GnRH浓素均低于对照区女性（p＜0.05）。携带ERα PvuII Pp,ERα XbaI Xx和ERα rs3798577T/T基因型的氟病区女性血清雌二醇浓度低于携带相同基因型的对照区女性（p＜0.05）。结论：ERα基因和氟暴露的交互作用可能影响女性血清生殖激素的浓度。

關鍵字

氟 ； ERα ； 基因多态性 ； 女性 ； 生殖激素

並列摘要

Background and Objectives: The aim of this study was to explore the influence of fluoride exposure and ERα gene polymorphisms on reproductive hormone concentrations of women in accordance with endemic fluorosis residence. Methods and Study Design: A cross sectional study was conducted in Tongxu county, Henan Province, China. A total of 679 women were recruited using cluster sampling and each subject provided fasting blood and an associated urine sample. We measured the concentrations of serum gonadotropin releasing hormone (GnRH), follicle-stimulating hormone, luteinizing hormone, and estradiol and urinary fluoride. Results: In the defluoridation project group (DFPG), serum GnRH was lower in women carrying C/C genotype compared to in those carrying C/T and T/T genotypes of ERα gene rs3798577 (p＜0.05). In the endemic fluorosis group (EFG), serum GnRH was lower in women carrying Pp genotype compared to in those carrying PP and pp genotypes of ERα PvuII (p＜0.05). Serum GnRH in women from EFG who carried Pp, pp, Xx and xx genotypes in ERα gene PvuII and XbaI was lower than in those in the control group (CG) who carried same genotypes (p＜0.05). Furthermore, serum GnRH in women from EFG was significantly lower than in those in CG, regardless of whether the women were carrying C/C, C/T or T/T genotypes of ERα rs3798577 (p＜0.05). Serum estradiol concentrations in EFG were significantly lower than in CG when the women were carrying the Pp, Xx and T/T genotypes in ERα gene (p＜0.05). Conclusion: Interaction of ERα gene and fluoride exposure may influence women's serum reproductive hormone concentrations.

並列關鍵字

fluoride ； ERα ； gene polymorphism ； women ； reproductive hormones