Title

Regulatory Analysis of IPP Isomerase Gene in Haematococcus pluvialis

Translated Titles

雨生红球藻中IPP异构酶基因表达调控机制的初步研究

Authors

高政权(Zheng-Quan Gao);孟春晓(Chun-Xiao Meng);叶乃好(Nai-Hao Ye)

Key Words

虾青素 ; 顺式作用元件 ; 5'上游侧翼学列 ; 雨生红球藻 ; IPP异构酶基因 ; astaxanthin ; cis-acting elements ; 5'-flanking region ; Haematococcus pluvialis ; IPP isomerase gene

PublicationName

Marine Science Bulletin

Volume or Term/Year and Month of Publication

11卷1期(2009 / 05 / 31)

Page #

37 - 42

Content Language

英文

Chinese Abstract

单细胞淡水绿藻-雨生红球藻能够在一定条件积累一种次生类胡萝卜素-虾青素,因而在生长后期藻体变为深红色。其中,IPP异构酶在雨生红球藻中的虾青素合成过程中发挥了重要作用。在本研究中,我们首次通过基因组上游步移克隆到了雨生红球藻中IPP异构酶基因的两个不同5'上游侧翼序列,大小分别是18kb和2.5kb。利用生物信息学方法分别对这两个不同5'上游侧翼序列进行了序列分析,结果发现,二者具有某些相同的顺式作用元件,如可能的脱落酸反应元件(ABRE)、干燥或低温反应元件(DRE/C-repeat)、几种光反应元件(G-box, GAG-motif, I-box and ATC-motif)、热激反应元件(HSE)、机械伤害反应元件(WUN-motif)、水杨酸反应元件(TCA-element)、生长素反应元件(TGA-element)、茉莉酸甲酯反应元件(TGACG-element)、缺氧特异反应中的增强子类似元件(GC-motif)和反式作用因子MYB蛋白的结合位点(MBS and MRE),但是二者并不具有典型的TATA框和CCAAT框。上述研究预示了雨生红球藻虾青素合成中IPP异构酶基因转录调控方式的多样化。

English Abstract

The unicellular green alga Haematococcus pluvialis uniquely accumulates carotenoids in the Cytoplasm and in late developmental stages turns deep-red in color because of accumulation of astaxanthin in the cytosol. The enzyme, isopentenyl pyrophosphate (IPP) isomerase, plays a key role in astaxanthin biosynthesis of H. pluvialis. In this paper, two separate 5-flanking regions (1.8 kb and 2.5 kb) of IPP isomerase gene was cloned through walking upstream firstly. Results of sequence analysis showed that two separate 5'-flanking regions of IPP isomerase gene might have similar putative cis-acting elements such as ABA (abscisic acid)-responsive element (ABRE), drought-responsive element (DREIC-repeat), light-responsive element (G-box, GAG-motif, I-box and ATC-motif), heat-shock element (HSE), wound-responsive element (WUN-motif), SA (salicylic acid)-responsive element (TCA-element), auxin-responsive element (TGA-element), MeJA (methyl jasmonate)-responsive element (TGACG-element), enhancer-like element involved in anoxic specific inducibility (GC-motif) and MYB binding sites (MBS and MRE), except for typical TATA box or CCMT box, which exhibit diversiform transcriptional patterns of IPP isomerase gene in astaxanthin biosynthesis of Haematococcus pluvialis.

Topic Category 基礎與應用科學 > 海洋科學