Cordyceps Sinensis Extract Promotes Phenotypic and Functional Maturation of Human Monocyte-Derived Dendritic Cells

冬蟲夏草提取液促進人類單核球轉化之樹突狀細胞的成熟與功能

魏麒容(Chi-Jung Wei) ； 郭敏玲(Ming-Ling Kuo) ； 楊榮季(Rong-Chi Yang) ； 張雪玲(Hsueh-Ling Chang) ； 謝佳蓉(Chia-Jung Hsieh)

《中醫藥雜誌》 16卷1期 (2005/03) Pp. 47-55

https://doi.org/10.6940/JCM.200503_16(1).04

摘要

Cordyceps sinensis (C. sinensis) is well known for its modulating effect on host immune system. As dendritic cells (DC) are professional antigen-presenting cells (APC) that play a key role in cancer immunotherapy, the maturation of DC is critical to the induction of T-cell responses. We investigated the effect of C. sinensis extract on the maturation of human monocyte-derived DC and the secretion of cytokines. Peripheral blood mononuclear cells (PBMC) from healthy donors were cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 6 days, then stimulated by hot-water extract of C. sinensis for 2 days. Stimulation with C. sinensis extract increased cell surface expression, and the RMFI (ratio of mean fluorescence intensity) of CD80, CD83, CD86 and HLA-DR are 1.70±0.32, 1.20±0.01, 2.29±0.29 and 1.44±0.23 (n=5, p＜0.05), respectively. Moreover, when co-cultured with allogeneic T cells, C. sinensis extract caused the significant production of IL-12 p40 (256.8±185.4 pg/mL, control group is 98.2±46.73 pg/mL) and IFN-γ (311.1±156.4 pg/mL, control group is 22.5±35.1 pg/mL). In conclusion, these findings suggest that C. sinensis extract is a potent stimulator of DC and may be related to modulation of Th1 cells functions.

關鍵字

冬蟲夏草 ； 樹突狀細胞 ； 協同刺激分子 ； T細胞 ； 中藥

並列摘要

Cordyceps sinensis (C. sinensis) is well known for its modulating effect on host immune system. As dendritic cells (DC) are professional antigen-presenting cells (APC) that play a key role in cancer immunotherapy, the maturation of DC is critical to the induction of T-cell responses. We investigated the effect of C. sinensis extract on the maturation of human monocyte-derived DC and the secretion of cytokines. Peripheral blood mononuclear cells (PBMC) from healthy donors were cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 6 days, then stimulated by hot-water extract of C. sinensis for 2 days. Stimulation with C. sinensis extract increased cell surface expression, and the RMFI (ratio of mean fluorescence intensity) of CD80, CD83, CD86 and HLA-DR are 1.70±0.32, 1.20±0.01, 2.29±0.29 and 1.44±0.23 (n=5, p＜0.05), respectively. Moreover, when co-cultured with allogeneic T cells, C. sinensis extract caused the significant production of IL-12 p40 (256.8±185.4 pg/mL, control group is 98.2±46.73 pg/mL) and IFN-γ (311.1±156.4 pg/mL, control group is 22.5±35.1 pg/mL). In conclusion, these findings suggest that C. sinensis extract is a potent stimulator of DC and may be related to modulation of Th1 cells functions.

並列關鍵字

Cordyceps sinensis ； Dendritic cells ； Co-stimulatory molecules ； T cells ； Herbal extract