A Preliminary Study of Phagocytotic Mechanism in Abalone (Haliotis diversicolor) Hemocyte

九孔血液細胞吞噬機制的初探

楊凱雯(Kai-Wen Yang) ； 陳俊宏(Jiun-Hong Chen)

《Acta Zoologica Taiwanica》 14卷2期 (2004/10) Pp. 121-122

https://doi.org/10.6576/AZT.2004.14.(2).16

摘要

本實驗對九孔血液細胞的吞噬機制作一多方向而初步的探討，發現1.血液細胞雖可在無血清的情況下進行吞噬作用，然而血清會增進吞噬作用達將近一倍。2.血液細胞可吞噬酵母菌，而醣類（mannan和laminarin）的存在，會有抑制作用，顯示血液細胞表面具有lectin可以辨認酵母菌細胞壁構成成分來協助吞噬反應。3.血液細胞也可以接合acLDL，表示可能會有Scavenger receptor的存在。另一方面，雖然細胞進行吞噬時其PKC活性沒有變化，而NO donor或NOS inhibitor對吞噬作用沒有影響，吞噬時的訊息傳導路徑以及ROS(reactive oxygen species)在九孔血液細胞吞噬機制中扮演的角色仍然有探討的空間。

關鍵字

九孔 ； 血液細胞 ； 吞噬作用 ； 血清 ； 酵母菌 ； 訊息傳導

並列摘要

In this study, three aspects of phagocytotic mechanisms in abalone (Haliotis diversicolor) hemocyte have been demonstrated. First, the hemocytes present both opnin-dependent or-independent phagocytosis. However, the plasma, which has putative opsonin in it, can enhance phagocytosis up to almost two times higher than that without plasma. Second, phagocytotic activity of abalone hemocytes to yeast can be inhibited by mannan or laminarin (components of yeast cell wall). It inferred that there may present lectins on hemocyte surface, which can enhance phagocytosis. Third, the hemocytes can phagocyte acLDL, which is a specific ligand of Scavenger receptor. Thus, it strongly suggested that hemocytes should have Scavenger receptors on their surfaces. On the other hand, so far, though we did not observe any difference in PKC activity during phagocytosis, or any change in phagocytotic behavior when treating the cell with NOS inhibitor or NO donor, it is worth to unravel the signalling pathway and the role of Nitrite Oxide on abalone hemocyte phagocytosis.

並列關鍵字

Haliotis diversicolor ； hemocyte ； phagocytosis ； lectin ； yeast ； signal transduction