- 期刊
- OpenAccess
Antioxidant Anti-Cancer and Antimicrobial Activities of Ethanol Pandanus amaryllifolius Roxb. leaf extract (In Vitro) - A potential medical application
摘要
Pandanus amaryllifolius (Roxb.) is a Southeast Asia tropical plant used in culinary and traditional medicine for century. It is a source of phytochemical constituents. The aim of the study was to evaluate the antimicrobial activity, antioxidant, and cytotoxic effect of the ethanol Pandanus amaryllifolius (Roxb.) leaf extracts for therapeutic potential in oral applications. To determine the amount of total phenolic contents and to evaluate the antioxidant activity of 70% ethanol leaf crude extracts of Pandanus amaryllifolius Roxb. the Folin-Ciocalteu method and the 2, 2-diphenyl-lpicrylhydrazyl (DPPH) assay were used. The ascorbic acid (vitamin C) and butylated hydroxytoluene (BHT) were used as standard positive controls. A broth serial micro dilution method was used to determine the minimum inhibitory concentration (MIC) against oral pathogens i.e. Porphyromonas gingivalis, Streptococcus mutans, Streptococcus sanguinis and Streptococcus salivarius compared to the standard antibiotics and 0.2% (w/v) chlorhexidine gluconate which were positive controls. The 3-(4,5-dimethylthiazolyl-2)-2,5- diphenyltetrazolium bromide reduction assay (MTT assay) was used for cytotoxicity effect tested. Results showed that the extracts contained 57.25 ± 0.02 mgGAE/g of total phenolic contents. The antioxidant activities of all tested substrates were calculated and exhibited as (IC50) the half maximum inhibitory concentration; is the concentration of antioxidant which reduces the free radical DPPH about 50%. The value of IC50 of ascorbic acid, the leaf extracts and BHT were 7.79 ± 1.25, 93.16 ± 10.46 and 110.57 ± 36.42 μg/mL,respectively. Pandanus amaryllifolius (Roxb.) leaf extracts had antimicrobial activities (MICs ranging from 32 to 125 μg/mL) μg/mL against tested oral bacteria. The extract exhibited the cytotoxicity effect on head and neck squamous cell carcinoma cell line (HNSCC cell line: HN31) with 50% lethal concentration (LC50) of 75.19 ± 3.48 μg/mL. Collective data from this study suggested the therapeutic potentials of the Pandanus amaryllifolius (Roxb.) for the future research and development health applications.
延伸閱讀
- Shaikh, R., Pund, M., Dawane, A., & Iliyas, S. (2014). Evaluation of Anticancer, Antioxidant, and Possible Anti-inflammatory Properties of Selected Medicinal Plants Used in Indian Traditional Medication. Journal of Traditional and Complementary Medicine, 4(4), 253-257. https://doi.org/10.4103/2225-4110.128904
- 陳彥卉、嚴玉芬、陳淑德（2021）。Study on Active Components and Antioxidant Activities of Poria cocos Solid-state Fermented Product Prepared from Soybean Residue and Rice Bran。臺灣農業化學與食品科學，59(4)，139-148。https://doi.org/10.6578/TJACFS.202112_59(4).0004
- 陳翊珠（2020）。Study on Antimicrobial and Antioxidant Activities by Synthesizing Nanoparticles Use Momordica cochinchinensis Spreng Leaf Extract〔碩士論文，國立屏東科技大學〕。華藝線上圖書館。https://doi.org/10.6346/NPUST202000279
- 余孟倫（2013）。Studies on the Chemical Constituents and Bioactivities from Aerial Part of Pandanus amaryllifolius〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://doi.org/10.6832/KMU.2013.00031
- Bationo, J. H., Hilou, A., Savadogo, P. W., & Nacoulma, O. G. (2012). Content of Polyphenolics Constituents and the Antioxidant and Antimicrobial Activities of Extracts from Leaves and Fruits of ＂Lannea microcarpa＂ Engl. & K. Kraus (Anacardiaceae). Current Research Journal of Biological Sciences, 4(3), 290-296. https://www.airitilibrary.com/Article/Detail?DocID=20410778-201204-201508180047-201508180047-290-296