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Partial Characterization of an Anti-Candida albicans Bacteriocin Produced by a Marine Strain of Bacillus sp., Sh10
並列摘要
The bacteriocin-producing strain Bacillus sp., Sh10, isolated from the marine environment, exhibited a broad spectrum of antimicrobial activity against different food spoilage and human pathogens, with a maximum inhibitory activity against Candida albicans. The inhibitory compound was sensitive to trypsin but resistant to proteinase K, lysozyme, lipase and α-amylase. It was heat-stable and remained its activity after autoclaving. In addition, the antimicrobial substance demonstrated striking stability at low temperatures (4 and -20°C) for up to one year and retained its activity in a wide pH range from 2 to 11. It was also stable and active in the presence of different surfactants, solvents and heavy metals. Analysis of the partially purified bacteriocin by SDS-PAGE showed an apparent molecular weight of ~11 KDa. This study reveals a remarkable potential of this bacteriocin to be used as a food preservative.
延伸閱讀
- Jayani, R. S., Shukla, S. K., & Gupta, R. (2010). Screening of Bacterial Strains for Polygalacturonase Activity: Its Production by Bacillus sphaericus (MTCC 7542). Enzyme Research, 2010(), 98-102. https://doi.org/10.4061/2010/306785
- Lin, P. C. (2010). Characterization of an arsenate-reducing bacterium Citrobacter sp. strain L2 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.10626
- 黃柏晨（2022）。Antibacterial effect of surfactin produced by Bacillus subtilis isolates on Staphylococcus〔碩士論文，國立屏東科技大學〕。華藝線上圖書館。https://doi.org/10.6346/NPUST202200062
- Suryanarayana, N., Vanlalhmuaka, Mankere, B., Verma, M., Thavachelvam, K., & Tuteja, U. (2016). Soluble Expression and Characterization of Biologically Active Bacillus anthracis Protective Antigen in Escherichia coli. Molecular Biology International, 2016(), 41-51. https://doi.org/10.1155/2016/4732791
- 曾子玲（2008）。Affinity purification of the Candida albicans Cdc4 associated proteins〔碩士論文，中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834/CSMU.2008.00060