Title

微流道表粗對於細胞貼附之影響

Translated Titles

Surface roughness effect on the cell attachment in a microchannel

DOI

10.6846/TKU.2012.00382

Authors

呂傑文

Key Words

微流道 ; PDMS ; 表面粗糙度 ; 細胞貼附 ; Microfluidic ; PDMS ; Surface roughness ; Cell attachment

PublicationName

淡江大學機械與機電工程學系碩士班學位論文

Volume or Term/Year and Month of Publication

2012年

Academic Degree Category

碩士

Advisor

楊龍杰

Content Language

繁體中文

Chinese Abstract

本研究以黃光微影技術和軟微影PDMS 翻模技術,製作出一個仿微血管 水力直徑的PDMS 微流道。先行驗證PDMS 在O2 與CF4 混合氣體不同比例下, 電漿蝕刻的表面粗糙度變化數據,再利用該不同表面粗糙度的PDMS 微流道, 透過微球體與活體肝癌細胞HepG2 之動態灌流實驗,分別觀察二者貼附於 PDMS 微流道之每隔10 分鐘變動的面積多寡,而至終定性歸納出,PDMS 微 流道表粗對於細胞貼附之影響。

English Abstract

A PDMS microchannel mimicking the capillary blood vessel is fabricated by the soft lithography. With treating the PDMS microchannels by different plasmas, the corresponding surface roughness RA data are experimentally measured. Using these PDMS microchannels well defined by proper RA values, liquid streams with microbeads and living HepG2 tumor cells are filled in, respectively. The microbead and cell attachment areas in the PDMS microchannel have been recorded dynamically with 10 min interval. Finally, the author qualitatively discusses the surface roughness effect on the particle or cell attachment in a PDMS microchannel.

Topic Category 工學院 > 機械與機電工程學系碩士班
工程學 > 機械工程
工程學 > 電機工程
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