Optimization of Enzymatic Bio-Fuel Cell for Immobilization of Glucose Oxidase on Chitosan Coated Carbon Cloth
酵素型生物燃料電池 ； 共價鍵結法 ； 反應曲面法 ； Enzymatic Bio-Fuel Cell ； Covalent ImmobilizingResponse Surface Methodology
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This study presents a high-performance biofuel cell based on the covalent immobilizing of glucose oxidase (GOx) on chitosan coated carbon cloth as an anodic catalyst. The chitosan was coated by the coagulation of an aqueous solution of chitosan on the carbon cloth surface. The N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) was used as coupling agents for GOx immobilization. The response surface methodology (RSM) and Box-Behnken design were employed to search the optimal immobilization conditions and understand the significance of the factors affecting the immobilized GOx activity. The results indicated that the pH, and the enzyme/support ratio are the statistically significant factors for GOx immobilization. In the ridge max analysis, the optimal immobilization conditions include a reaction time of 50 min, a pH of 5.9, and an enzyme/support ratio of 3 (w/w). Under the optimal condition, the predicted and the experimental immobilized GOx activities were 34.42±1.07 and 33.50±0.92 U/g-support, respectively. Based on the regression model, the carbon cloths with various GOx activities were prepared, and the GOx activity effect on the power density generated from the biofuel cell was investigated. The power density was increased with GOx activity, and the maximum power 1.672 mW/cm2 was obtained at a cell voltage of 0.44 V.
工程學 > 工程學總論