Title

國蘭專一性培養基營養元素組成份開發及垂頭地寶蘭開花習性與種子繁殖

Translated Titles

Development of Nutrient Elements Composing in Specific Medium of Chinese Cymbidium and Flowering Habitual Behavior and Seed Propagation of Geodorum densiflorum (Lam.) Schltr.

DOI

10.6845/NCHU.2012.00875

Authors

許芸榕

Key Words

國蘭 ; 垂頭地寶蘭 ; 營養 ; 組織培養 ; 培養基 ; Chinese Cymbidium ; Geodorum densiflorum (Lam.) Schltr. ; Medium ; Tissue culture

PublicationName

中興大學園藝學系所學位論文

Volume or Term/Year and Month of Publication

2012年

Academic Degree Category

碩士

Advisor

張正

Content Language

繁體中文

Chinese Abstract

使用玉花四季蘭(Cymbidium ensifolium `Yuh Hwa')、觀音素心蘭(Cymbidium ensifolium `Misericors')及宋梅春蘭(Cymbidium goeringii `Song Mei')進行葉片分析,將分析所得之元素組成換算成培養基鹽類組成,培養對應的根莖。根莖增殖培養基培養之根莖直徑與鮮重都顯著優於對照組。玉花四季蘭的根莖抽芽培養基可分化出具展開葉的植株,觀音素心蘭培養於根莖增殖培養基中經過長時間會分化出植株,在抽芽培養基中反而只能長出短芽體。   垂頭地寶蘭(Geodorum densiflorum (Lam.) Schltr.)為臺灣原生蘭之一,四月中開始抽出葉芽,六月底開始開花,抽芽至開花所需時間短,可利用其抽芽至開花時間短之特性發展成為觀賞花卉。垂頭地寶蘭以授粉後6個月之裂果種子播種有較高之發芽率及較平均之發芽勢,培養根莖以全量MS基礎培養基為佳,而根莖抽芽較適培養基為半量或全量MS基礎培養基,根莖成熟度會影響產生之植株的健康程度。   垂頭地寶蘭的組織培養與國蘭相似,同樣是先形成根莖後再抽芽,但從無菌播種至形成植株僅需一年左右,效率高於親緣相近的國蘭,且使用前人開發之培養基,即可達到此效果。若將國蘭從初代至器內培養過程中皆使用調整後之培養基,或掌握國蘭生長週期中之營養變化,可能將其培養時間縮短,得到較高之效率。

English Abstract

Analysis the elements in leaves of Yuh Hwa, Misericors and Song Mei, and converse the elementary components to the salt components of medium, to culture the corresponding rhizomes. The diameter and fresh weight of rhizomes in the initiation media are better than the control media. It can sprout shoots with spreading leaves in the shooting media of Yuh Hwa, and it can sprout shoots in rhizome initiation media of Misericors, but in the shooting media only produce short bud. Geodorum densiflorum (Lam.) Schltr. is one of protogenic orchids in Taiwan. They start sprouting shoots from rhe mid-April, and start flowering at the end of June. It needs short time from sprouting to flowering, so it is great to develop into ornamental flowers. The seeds in spilt capsules at pollination after 6 months have higher germination percentage and equal germination potential in Geodorum densiflorum (Lam.) Schltr. It is good to initiate the rhizomes of full strength MS basal medium, and good to sprout shoots in full strength or half strength MS basal media. The mature of rhizomes will affects the health of the shoots producing from rhizomes. It is similar to the tissue culture of Geodorum densiflorum (Lam.) Schltr. and Chinese Cymbidium. Before shooting, it forms rhizomes. From asymbiotic seed germination to the formation of plantlet of Geodorum densiflorum (Lam.) Schltr. needs only about one year. The efficiency is higher than the similar phylogenetic relationship Chinese Cymbidium. And Geodorum densiflorum (Lam.) Schltr. can achieve this effect in reference media. If use the modified media from the primary culture to formation of plantlet in Chinese Cymbidium, may shorten the time of culture, to achieve the higher effect.

Topic Category 農業暨自然資源學院 > 園藝學系所
生物農學 > 農業
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