Title

不同沙門氏菌血清型之沙門氏菌基因島分子流行病學研究

Translated Titles

Molecular Epidemiology of Salmonella Genomic Island 1 (SGI1) in Various Salmonella Serovars

DOI

10.6845/NCHU.2013.00833

Authors

蕭依玲

Key Words

沙門氏菌 ; 血清型 ; 沙門氏菌基因島 ; 多重抗藥性 ; Salmonella ; Serovars ; Salmonella Genomic Island 1 (SGI1) ; Multidrug resistance

PublicationName

中興大學微生物暨公共衛生學研究所學位論文

Volume or Term/Year and Month of Publication

2013年

Academic Degree Category

碩士

Advisor

張照勤

Content Language

繁體中文

Chinese Abstract

多重抗藥性沙門氏菌之重要性一直是全球性公共衛生的關注議題。沙門氏菌基因島(Salmonella genomic island 1;SGI1)中包含多重抗藥基因區域,並首次在多重抗藥Salmonella Typhimurium DT104流行中被證實,且對於五大類之抗生素產生抗藥性,分別為ampicillin (A)、chloramphenicol (C)、 streptomycin (S)、sulfonamide (Su)和tetracycline (T)。至今除沙門氏菌基因島原型外,已在不同沙門氏菌血清型及奇異變型桿菌(Proteus mirabilis)中被證實有SGI1-A至SGI1-V之變體。本研究目的為收集在台灣分離之9種不同沙門氏菌血清型菌株攜帶SGI1盛行率分佈並發展一個快速將SGI1原型和變體分型的分子診斷工具。結果顯示有22.9%沙門氏菌攜帶SGI1,而在SGI1陽性率於人和動物來源的比較結果顯示,36.7%(69/188)人類分離株為SGI1陽性率較動物分離株中的陽性率13.5%(37/275)在統計學上顯著為高(p<0.05)。不同SGI1變體被辨別在不同血清型中,分別為:S. Typhimurium有攜帶SGI1原型、S. Choleraesuis為攜帶SGI1-J3變體、S. Albany為SGI1-B和SGI1-F之變體、S. Newport為SGI1-F和SGI1-I變體以及S. Bredeney之菌株有攜帶SGI1-F,而其他血清型如:S. Enteritidis、S. Heron、S. Treforest和S. 4,[5],12:i:-則沒有SGI1之存在。然而在S. Enteritidis中雖然沒有SGI1存在,但卻有50%菌株呈現多重抗藥性的表現。另,SGI1在分子分型結果與抗藥型別之比對分析後發現,一些特殊的SGI1並不一定為固定的多重抗藥表現。就我們所知道的研究中,本研究為國際中首次發現在S. Choleraesuis中攜帶SGI1-J3和S. Bredeney中攜帶SGI1-F之變體。值得一提的是在S. Choleraesuis血清型發現一個攜帶相似SGI1-J6變體對於CSSuT抗藥之菌株,其在第一個整合子呈現intI1- sat- psp- aadA2- qacEΔ1- sul1之基因結構,並在SGI1骨架的ORF S023中沒有發現ISSen5序列之插入,因此此菌株所攜帶的SGI1變體稱之為SGI1-J6 like。

English Abstract

Multidrug resistance (MDR) in Salmonella has become a global public health issue. Salmonella genomic island 1 (SGI1) was initially described in the epidemic Salmonella Typhimurium phage type DT104 strain and contains a MDR region with resistance of ampicillin (A), chloramphenicol (C), streptomycin (S), sulfonamide (Su) and tetracycline (T). Variants of SGI1 from SGI1-A to SGI1-V have been described in different Salmonella serovars and Proteus mirabilis. The objective of this study was to develop a quick molecular method to identify SGI1 variants in 463 Salmonella isolates including 9 serovars in Taiwan. The results indicated that 22.9% of Salmonella isolates carrying SGI1, and prevalence of SGI1 positivity in human isolates were significantly higher than that in animal isolates (36.7% vs. 13.5%; p<0.05). Different SGI1 variants were identified in various Salmonella serovars: SGI1 in S. Typhimurium, SGI1-J3 in S. Choleraesuis, SGI1-B and SGI1-F in S. Albany, SGI1-F and SGI1-I in S. Newport and SGI1-F in S. Bredeney. None of the S. Enteritidis, Heron, Treforest and Salmonella serovar 4,[5],12:i:- were SGI1-positive. However, MDR of ACSSuT could still be observed in S. Enteritidis. It was also found that the isolate with the specific SGI1 variant did not always show a unique MDR phenotype. To the best of our knowledge, this is the first study to identify SGI1-J3 in S. Choleraesuis and SGI1-F in S. Bredeney. Furthermore, in S. Choleraesuis, we identified one novel SGI1 variant showing CSSuT resistance. This variant is closest to SGI1-J6 but with the gene cassette of intI1- sat- psp- aadA2- qacEΔ1- sul1 in the first fragment of class I integron and without insertion of ISSen5 within ORF S023 in SGI1 backbone.

Topic Category 醫藥衛生 > 基礎醫學
醫藥衛生 > 預防保健與衛生學
獸醫學院 > 微生物暨公共衛生學研究所
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