Title

苦茶粕防治甘藍幼苗立枯病之效果與其有效成分之鑑定

Translated Titles

Effect of tea seed pomace on control of cabbage seedling damping-off caused by Rhizoctonia solani AG-4 and identification for its major ingredient of antifungal activity

DOI

10.6845/NCHU.2006.00601

Authors

楊正偉

Key Words

甘藍 ; 立枯絲核菌 ; 苦茶粕 ; 萃取 ; cabbage ; Rhizoctonia solani ; tea seed pomace ; extract

PublicationName

中興大學植物病理學系所學位論文

Volume or Term/Year and Month of Publication

2006年

Academic Degree Category

碩士

Advisor

黃振文

Content Language

繁體中文

Chinese Abstract

立枯絲核菌(Rhizoctonia solani kühn AG-4)可以引起甘藍幼苗猝倒、萎凋與死亡的現象,是甘藍苗床栽培的主要病原菌之一。使用種子誘釣法篩選具有抑制R. solani纏據甘藍種子效果的有機添加物,結果發現BVB No.4 泥炭苔中添加2%(w/v)苦茶粕處理當天即可降低種子纏據百分率達68%,處理一星期後,也可得到類似的效果。添加1%(w/v)苦茶粕可降低甘藍苗立枯病的發病率達40%左右。進一步探討苦茶粕防病的原理,發現添加1%(w/v)苦茶粕於BVB No.4 泥炭苔中,會提高微生物族群量,其中細菌菌量上升9倍,真菌及放線菌則分別增加2~3倍,但對BVB No.4 泥炭苔pH值影響不大。去除BVB No.4 泥炭苔中的微生物,發現苦茶粕仍能降低R. solani纏據種子百分率30%以上。以5倍體積甲醇在室溫下萃取苦茶粕,進一步使用管柱純化後,依Liebermann-Burchard反應確定其為三萜類皂素。在馬鈴薯葡萄糖瓊脂培養基中添加1%(w/v)苦茶粕粗萃取液,其抑菌百分率即可達50%。當無菌水中添加1%(w/v)苦茶粕粗萃取液時,將菌絲團塊置入後,可發現細胞通透性改變,到第6天導電度可以上升60μs,惟以無菌水處理菌絲團塊其導電度未明顯沒有上升。在影響苦茶粕抑菌性之因子方面,基質的pH值改變並不影響苦茶粕有效性:然而添加氮肥卻會降低苦茶粕防治甘藍立枯病的效果。

English Abstract

Damping-off disease caused by Rhizoctonia solani kühn AG-4 is a serious disease of cabbage seedlings especially in culture medium. Tea seed pomace was added(2%,w/v)in BVB NO.4 peat moss medium infested with R. solani AG-4. It was significantly effective in inhibiting colonization of cabbage seed caused by R. solani AG-4 at 0 and 7th days after treatment. Tea seed pomace at the rate of 1%(w/v)was able to markedly reduce the disease incidence of cabbage seedling damping-off by 40% compared to non-treatment as a control. Tea seed pomace could stimulate proliferation of beneficial microorganisms including fungi, bacteria and actinomycetes. The pH values did not change significantly after tea seed pomace was added(1%,w/v)in BVB NO.4 peat moss medium. The methnol-extractive compound was obtained from purification procedure of Diaion HP-20column chromatography and acetone fraction. The compound was characterized by Liebermann-Burchard reaction as triterpenoid saponin. The methnol-extractives were added(1%,w/v)in potato dextrose agar for evaluating their effect on mycelial growth of R. solani AG-4. The mycelial growth was markedly inhibited higher than non-treatment about 50%. Amendment of NH4NO3 or Urea was able to nullify tea seed pomace to reduce damping-off disease incidence of cabbage seedlings caused by R. solani AG-4.

Topic Category 農業暨自然資源學院 > 植物病理學系所
生物農學 > 植物學
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Times Cited
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