Title

2007-2008年間台灣鸚鵡喙羽病之盛行率調查及病毒核酸序列分析

Translated Titles

The Prevalence of Psittacine Beak and Feather Disease (PBFD) and Viral Genomic Sequences Analysis in Taiwan during 2007-2008

DOI

10.6845/NCHU.2009.01075

Authors

官南綾

Key Words

鸚鵡喙羽病 ; 盛行率調查 ; 環狀病毒 ; 序列分析 ; Psittacine ; Beak and Feather Disease Virus (BFDV) ; circovirus ; sequences analysis

PublicationName

中興大學獸醫學系暨研究所學位論文

Volume or Term/Year and Month of Publication

2009年

Academic Degree Category

碩士

Advisor

沈瑞鴻

Content Language

繁體中文

Chinese Abstract

鸚鵡喙羽病病毒( Beak and feather disease virus, BFDV)屬於環狀病毒(Circoviridae)科環狀病毒屬(Circovirus),其感染鸚鵡科鳥類所造成之臨床症狀包括有羽毛發育不良、脫落甚至引發免疫抑制,部分鳥種會出現喙部及爪之病變,故得名鸚鵡喙羽病(Psittacine beak and feather disease, PBFD)(Perry et al., 1981)。2002-2005年間,台灣之研究顯示籠飼鸚鵡之陽性率為41.2% (68/165),顯示鸚鵡喙羽病之存在(Hsu et al., 2006),本研究依此為出發點,進而希望了解台灣鸚鵡繁殖場之盛行率及病毒演化之情形。研究期間為2007-2008年並分成三部分:首先針對PBFDV之ORFV1 (Rep) 序列設計一引子對,以PCR之方式對於鸚鵡隻羽毛、糞便檢進行檢測。採樣源自台灣東部、中部及南部之3個鳥園、5個鸚鵡繁殖場及臨床病例,共計1285個檢體,其鸚鵡種類包含40科95屬。在臨床病例中,陽性率為 64.3% (27/42),在鳥園的部分為 5.9% (28/474) 到36.4% (4/11),而繁殖場為29.2%(33/113)到76.3% (116/152)。整體陽性率則為 31.8% (408/1285),顯示鸚鵡喙羽病已普遍存在於台灣。將1285個檢體的資料詳細記錄,包含以下五個變項:地區(Location)、來源型態(Type)、起源(Source)、年齡(Age)及臨床症狀(Clinical sign);以多變項邏輯式迴歸分析校正所有可能之干擾因素計算調整後危險勝算比(adjusted odds ratio;aOR),在地區分布方面南部之陽性率最高(aOR=8.15,95% CI=3.90-17.00);在檢體來源方面是以臨床病例陽性率最高(aOR=7.18,95% CI=3.09-17.00);鳥種起源方面是舊世界鳥種比新世界鳥種有較高之陽性率(aOR=4.01,95% CI=2.61-6.16);在年齡方面是雛鳥-亞成鳥之陽性率高於年輕成鳥-成鳥(OR=1.33,95% CI=0.67-2.67),但在校正後兩者間之陽性率不具統計學上之意義(p=0.42>0.05);在臨床症狀方面,羽毛異常者比外觀正常者有較高之陽性率(aOR=79.00,95% CI=28.98-215.32)。第二部分則收集陽性鸚鵡,每個月皆採取羽毛及泄殖腔拭子展開為期兩年之長期排毒監測,14隻陽性鸚鵡除了中途死亡之5隻鸚鵡外,其餘9隻鸚鵡至實驗結束止,兩種檢體皆為BFDV核酸陽性。中途死亡之鸚鵡則採全身臟器進行病毒分布調查,所有採集之臟器皆可測到BFDV之核酸,並且可能終身排毒。第三部分是病毒序列分析,將17株全長序列與GeneBank現有之序列,針對ORF V1、ORF C1及全長相較之間親源性及基因演化;4株序列(PsiE-TWe07、Ecl-TWe08、Cos-TWe09及Tri-TWc13)源自南非具有地區性,8株(Aga -TWs01~ TWs03、Mel-TWs04~ TWs06、Mel-TWs17及PsiE-TWc12)具有鳥屬特性,5株(Cac-TWc11、Cac-TWc10、Cac-TWc14、Cac-TWn16及Pseu-TWs15)與地區或鳥屬無明顯統一性,尚無出現台灣特有之序列。

English Abstract

Psittacine beak and feather disease (PBFD) is a progressive symmetric feather dystrophy, which is caused by a circular, single-strand DNA virus belonging to Circoviridae Circovirus. Since 1981, the first PBFD case was described in Australia, over 60 species of psittacine birds are susceptible for this disease. Epidemiological researches and case reports were announced from many countries, including United States, United kingdom, Italy and Germany, showing that BFDV might be transmitted worldwide through international trade. In Taiwan, a previous study based on clinical cases revealed that positive rate was 41.2% (68/165) during 2002-2005 (Hsu et al., 2006). In our study, we collected feathers and feces from clinical cases, 3 bird parks and 5 independent breeding facilities, 1285 birds in total including 40 genera and 95 species during 2007-2008. Basing on the sequences of the ORFV1 (Rep) of BFDV, we designed a primer set for PCR detection, and the expected amplicon size was 420 bp. In conclusion, the positive rate was 64.3% (27/42) in clinical cases, and the positive rate in breeding facilities ranged from 5.9% (28/474) to 76.3% (116/152). The total positive rate was 31.8% (408/1285), indicating that BFDV persistently existed in Taiwan. In statistic analysis, the variables were including Location (eastern, southern and central), Type (bird park, breeding facility and clinical case), Source (new world and old world genera), Age (young adult-adult and nestling-juvenile) and Clinical sign (normal and feather dystrophy); The results of multiple logistic regression showed that subjects from southern Taiwan (aOR=8.15, 95% CI=3.90-17.00), clinical cases (aOR=7.18, 95% CI=3.09-17.00), old world genera (aOR=4.01, 95% CI=2.61-6.16) and feather dystrophy (aOR=79.00, 95% CI=28.98-215.32) were significantly associated with PCR-positive rate; In the second part of our study, 14 BFDV positive birds were tested for the long-term (24 months) shedding, the feather and cloacal swab were collected every month. 9 birds were PCR positive in both samples at the end of the experiment; 5 birds died halfway were tested for the BFDV distribution in organs, all the collected organs were PCR positive. In the final part, alignment of 17 isolates in our study with 41 sequences from GeneBank, 4 isolates were clustered with South Africa isolates (AY450438-442); 8 isolates were clustered with groups of psittacine species indicating genotypic association between the viruses and the host, but 4 isolates from cockatoos and 1 from dusky lorikeet did not show the species differences. The established phylogenetic tree did not support the existence of specific genotypes in Taiwan.

Topic Category 獸醫學院 > 獸醫學系暨研究所
生物農學 > 獸醫
Reference
  1. 柯靜怡。鸚鵡Avian polyomavirus及Psittacine beak and feather disease viruse感染症之病毒核酸序列分析。國立台灣大學碩士論文。2004。
    連結:
  2. Albertyn J, Tajbhai KM, and Bragg RR. Psittacine beak and feather disease virus in budgerigars and ring-neck parakeets in South Africa. Onderstepoort J Vet Res 71: 29-34, 2004.
    連結:
  3. Baker JR. Survey of feather diseases of exhibition budgerigars in the United Kingdom.Vet Rec 139: 590-594, 1996.
    連結:
  4. Bassami MR, Berryman D, Wilcox GE, and Raidal SR. Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus. Virology 249: 453-459, 1998.
    連結:
  5. Bassami MR, Ypelaar I, Berryman D, Wilcox GE and Raidal SR. Genetic diversity of beak and feather disease virus detected in psittacine species in Australia. Virology 279: 392-400, 2001.
    連結:
  6. Bert E, Tomassone L, Peccati C, Navarrete MG and Sol SC. Detection of beak and feather disease virus (BFDV) and avian polyomavirus (APV) DNA in psittacine birds in Italy. J Vet Med 52: 64–68, 2005.
    連結:
  7. Bonne N, Shearer P, Sharp M, Clark P, and Raidal S. Assessment of recombinant beak and feather disease virus capsid protein as a vaccine for psittacine beak and feather disease. J Gen Virol 90: 640–647, 2009.
    連結:
  8. Crowther R A, Berriman JA, Curran WL, Allan GM, and Todd D. Comparison of the structures of three circoviruses: chicken anemia virus, porcine circovirus type 2, and beak and feather disease virus. J Virol 77: 13036–13041, 2003.
    連結:
  9. Dean FB, Nelson JR, Giesler TL and Lasken RS. Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification. Genome Res 11: 1095-1099, 2001.
    連結:
  10. Doneley RJ. Acute beak and feather disease in juvenile African Grey parrots--an uncommon presentation of a common disease. Aust Vet J.81: 206-207, 2003.
    連結:
  11. Gelderblom H, Kling S, Lurz R, Tischer I, and von Bulow V. Morphological characterization of chicken anaemia agent (CAA). Arch Virol 109: 115-120, 1989.
    連結:
  12. Gorski F. DNA replication: the rolling circle model; critical remarks. Bull Acad Pol Sci Biol 23: 785-792, 1975.
    連結:
  13. Ha HJ, Anderson IL, Alley MR, Springett BP, and Gartrell BD. The prevalence of beak and feather disease virus infection in wild populations of parrots and cockatoos in New Zealand. N Z Vet J 55: 235-238, 2007.
    連結:
  14. Hattermann K, Schmitt C, Soike D, and Mankertz A. Cloning and sequencing of Duck circovirus (DuCV). Arch Virol 148: 2471-2480, 2003.
    連結:
  15. Heath L, Martin DP, Warburton L, Perrin M, Horsfield W, Kingsley C, Rybicki EP and Willianson A. Evidence of unique genotypes of beak and feather disease virus in South Africa. J Virol 78: 9277-9284, 2004.
    連結:
  16. Heath L, Williamson A, and Rybicki EP. The Capsid protein of beak and feather disease virus binds to the viral DNA and is responsible for transporting the replication-associated protein into the nucleus. J Virol 80: 7219-7225, 2006.
    連結:
  17. Hess M, Scope A and Heincz U. Comparitive sensitivity of polymerase chain reaction diagnosis of psittacine beak and feather disease on feather samples, cloacal swabs and blood from budgerigars (Melopsittacus undulates, Shaw 18005). Avian pathol 33: 477-481, 2004.
    連結:
  18. Hsu CM, Ko CY, and Tsai HJ. Detection and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from psittacine birds in Taiwan. Avian Dis 50: 348–353, 2006.
    連結:
  19. Jergens AE, Brown TP, and England TL. Psittacine beak and feather disease syndrome in a cockatoo. J Am Vet Med Assoc 193: 1292-1294, 1988.
    連結:
  20. Johne R, Raue R, Grund C, Kaleta EF, and Müller H. Recombinant expression of a truncated capsid protein of beak and feather disease virus and its application in serological tests. Avian Pathol 33: 328-336, 2004.
    連結:
  21. Kiatipattanasakul-Banlunara W, Tantileartcharoen R, Katayama K, Suzuki K, Lekdumrogsak T, Nakayama H, and Doi K. Psittacine beak and feather disease in three captive sulphur-crested cockatoos(Cacatua galerita) in Thailand. J Vet Med Sci 64: 527-529, 2002.
    連結:
  22. Katoha H, Ohyab K, and Fukushia H. Development of novel real-time PCR assays for detecting DNA virus infections in psittaciform birds. J Vet Med 154: 92-98, 2008.
    連結:
  23. Khalesi B, Bonne N, Stewart M, Sharp M and Raidal S. A comparison of haemagglutination, haemagglutination inhibition and PCR for the detection of psittacine beak and feather disease virus infection and a comparison of isolates obtained from loriids. J Gen Virol 86: 3039-3046, 2005.
    連結:
  24. Kloet E and Kloet S R. Analysis of the beak and feather disease viral genome indicates the existence of several genotypes which have a complex psittacine host specificity. Arch Virol 149: 2393-2412, 2004.
    連結:
  25. Kondiah K, Albertyn J, and Bragg RR. Beak and feather disease virus haemagglutinating activity using erythrocytes from African Grey parrots and Brown-headed parrots. Onderstepoort J Vet Res 72: 263-5, 2005.
    連結:
  26. Kondiah K, Albertyn J, and Bragg RR. Genetic diversity of the Rep gene of beak and feather disease virus in South Africa. Arch Virol 151: 2539-2545, 2006.
    連結:
  27. Latimer K S, Rakich PM, Niagro FD, Ritchie BW, Steffens W L, and Lukert PD. Extracutaneous viral inclusions in psittacine beak and feather disease. J Vet Diagn Invest 2: 204-207, 1990.
    連結:
  28. Latimer K S, Rakich PM, Kircher IM, Ritchie BW, Niagro FD, Steffens W L, Campagnoli RP, Pesti D and Lukert PD. An update review of psittacine beak and feather disease. J Assoc avian Vet 5: 211-220, 1991.
    連結:
  29. Lindahl T and Nyberg B. Heat-induced deamination of cytosine residues in deoxyribonucleic acid. Biochem 13: 3405-3410, 1974.
    連結:
  30. Liu Q, Tikoo SK, Babiuk LA. Nuclear localization of the ORF2 protein encoded by porcine circovirus type 2. Virology. 285: 91-99, 2001.
    連結:
  31. Lukert PD, de Boer GF, Dale JL, Keese P, McNulty MS, Randles JW, and Tischer I. The Circoviridae. In Virus Taxonomy. Classification and Nomenclature of Viruses. Sixth Report of the International Committee of Taxonomy of Viruses. F. A. Murphy, C. M. Fauquet, D. H. L. Bishop, S. A. Ghabrial, A. W. Jasivs, G. P. Martelli, M. A. Mayo, M. D. Summers (eds.). Springer-Verlag, New York, USA, 166-168, 1995.
    連結:
  32. Mankertz A, Hattermann K, Ehlers B, and Soike D. Cloning and sequencing of columbid circovirus (CoCV), a new circovirus from pigeons. Archives of Virology 145: 2469-2479, 2000.
    連結:
  33. McNulty MS. Chicken anaemia agent: a review. Avian Pathology 20: 187-203, 1991.
    連結:
  34. McOrist S, Black DG, and Pass DA. Psittacine beak and feather dystrophy in wild Sulphur-crested cockatoos (Cacatua galerita). J Wildl Dis 20: 120-124, 1984.
    連結:
  35. Niagro FD, Forsthoefel AN, Lawther RP, Kamalanathan L, Ritchie BW, Latimer KS, and Lukert PD. Beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. Arch Virol 143: 1723-1744, 1998.
    連結:
  36. Ogawa H, Yamaguchi T, and Fukushi H. Duplex shuttle PCR for differential diagnosis of budgerigar fledgling disease and psittacine beak and feather disease. Microbiol Immunol 49: 227-237, 2005.
    連結:
  37. Pass DA and Perry RA. The pathology of psittacine beak and feather disease. Aust Vet J 61: 69-74,1984.
    連結:
  38. Pass DA, Plant SL and Sexton N. Natural infection of wild doves (Streptopelia senegalensis). Aust Vet J 71: 307-308, 1994.
    連結:
  39. Phenix K V, Weston JH, Ypelaar I, Lavazza A, Smyth JA, Todd D, Wilcox GE, and Raidal SR. Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae. J Gen Virol 82: 2805–2809, 2001.
    連結:
  40. Posada D, Crandall KA and Holmes EC. Recombination in evolutionary genomics. Annu Rev Genet 36: 75-97, 2002.
    連結:
  41. Pringle CR. Virus taxomony at the XIth International Congress of Virology, Sydney, Australia, 1999. Arch Virol 144: 2065-2070, 1999.
    連結:
  42. Rahaus M and Wolff MH. Psittacine beak and feather disease: a first survey of the distribution of beak and feather disease virus inside the population of captive pstittacine bird in Germany. J Vet Med B 50: 368-371, 2003.
    連結:
  43. Raidal SR, Sabine M, and Cross GM. Laboratory diagnosis of psittacine beak and feather disease by haemagglutination and haemagglutination inhibition. Aust Vet J 70: 133-137, 1993a.
    連結:
  44. Raidal SR, McElnea CL, and Cross GM. Seroprevalence of psittacine beak and feather disease in wild psittacine birds in New South Wales. Aust Vet J 70: 137-139, 1993b.
    連結:
  45. Raidal SR, Cross GM. The haemagglutination spectum of psittacine beak and feather disease virus. Avian Pathology 23:621-630, 1994.
    連結:
  46. Ramis A, Latimer KS, Niagro FD, Campagnoli RP, Ritchie BW, and Pesti D. Diagnosis of psittacine beak and feather disease(PBFD) viral infection, avian polyomavirus infection, adenovirus infection and herpesviros infection in psittacine tissues using DNA in situ hybridization. Avian Pathol 23: 643-57, 1994.
    連結:
  47. Raue R, Johne R, Crosta L, Bürkle M, Gerlach H, and Müller H. Nucleotide sequence analysis of Cl gene fragment of psittacine beak and feather disease virus amplified by real-time polymerase chain reaction indicates a possible existence of genotype. Avian Pathol 33: 41-50, 2004.
    連結:
  48. Ritchie BW, Niagro FD, and Lukert PD. Characterization of a new virus from cockatoos with psittacine beak and feather disease. Virology 171: 83-88, 1989.
    連結:
  49. Ritchie BW, Niagro FD, Latimer KS, Lukert PD, Steffens WL, Rakich PM, and Pritchard N. Ultrastructural, protein composition, and antigenic comparison of psittacine beak and feather disease virus purified from four genera of psittacine birds. J Wildl Dis 26: 196-203, 1990.
    連結:
  50. Ritchie BW, Niagro FD, Latimer KS, Steffens WL, Pesti D, Ancona J, and Lukert D. Routes and prevalence of shedding of psittacine beak and feather disease virus. Am J Vet Res 53: 1804-1809, 1991b.
    連結:
  51. Ritchie PA, Anderson IL, andLambert DavidM. Evidence for specificity of psittacine beak and feather disease viruses among avian hosts. Virology 306: 109-115, 2003.
    連結:
  52. Sanada Y, Sanada N and Kubo M. Electron Microscopical Observations of Psittacine Beak and Feather Disease in an Umbrella Cockatoo (Cacatua alba). J Vet Med Sci 61: 1063–1065, 1999.
    連結:
  53. Sanada N and Sanada Y. The sensitivities of various erythrocytes in a haemagglutination assay for the detection of psittacine beak and feather disease virus. J Vet Med 47: 441–443, 2000.
    連結:
  54. Sexton N, Penhale WJ, Plant SL, and Pass DA. Use of goose red blood cells for detection of infection with psittacine beak and feather disease virus by haemagglutination and haemagglutination inhibition. Aust Vet J 71: 345-347, 1994.
    連結:
  55. Shearer PL, Bonnea N, Clark P, Sharp M, and Raidal SR. Development and applications of a monoclonal antibody to a recombinant beak and feather disease virus (BFDV) capsid protein. J Virol Methods 147: 206–212, 2008.
    連結:
  56. Stewart M E, Bonnea N, Shearer P, Khalesi B, Sharp M, and Raidal S. Baculovirus expression of beak and feather disease virus (BFDV) capsid protein capable of self-assembly and haemagglutination. J Virol Methods 141: 181–187, 2007.
    連結:
  57. Tischer I, Peters D, Rasch R, and Pociuli S. Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch Virol 96: 39-57, 1987.
    連結:
  58. Todd D, Niagro FD, Ritchie BW, Curran W, Allan GA, Lukert PD, Latimer KS, Steffens III WL, McNulty MS. Comparison of three animal viruses with circular single-stranded DNA genomes. Arch Virol 117: 129-135, 1991
    連結:
  59. Todd D. Circoviruses: immunosuppressive threats to avian species: a review. Avian Pathol 29: 373-394, 2000.
    連結:
  60. Todd D, Weston JH, Soike D, and Smyth JA. Genome sequence determinations and analyses of novel circoviruses from goose and pigeon. Virology 286: 354-362, 2001.
    連結:
  61. Trinkaus K, Gravendyck M, Leiser R, Wenish S and Kaleta EF. Psittacine beak and feather disease infected cells show a pattern of apoptosis in psittacine skin. Avian Pathol 27: 555-561, 1998.
    連結:
  62. Woods LW, Latimer KS, Barr BC, Niagro FD, Campagnoli RP, Nordhausen RW and Castro AE. Circovirus-like infection in a pigeon. J Vet Diagn Invest 5: 609-612, 1993.
    連結:
  63. Wylie SL and Pass DA. Experimental reproduction of psittacine beak and feather disease/ French moult. Avian Pathol 16: 269-281, 1987.
    連結:
  64. Ypelaar I, Bassami MR, Wilcox GE, and Raidal SR. A universal polymerase chain reaction for the detection of psittacine beak and feather disease virus. Vet Microbiol 68: 141-148, 1999.
    連結:
  65. 蔡錦文。鸚鵡圖鑑、自然珍藏系列26。貓頭鷹出版社。2005。
  66. Demidov VV. Rolling-circle amplification(RCA). In: Encyclopedia of diagnostic genomics and proteomics. Marcel Dekker, Inc., New York, USA, 1175-1179, 2005.
  67. Goldsmith TL. Documentation of passerine circoviral infection. Proceedings of Association for Avian Veterinary 16: 349, 1995.
  68. Perry RA. A psittacine beak and feather disease syndrome, in Proceedings, No.55 of Courses for Veterinarians. Cage and aviary birds. Sydney, Australia: The Post Graduate Committee in Veterinary Science 55: 81-108, 1981.
  69. Ritchie BW, Niagro FD, Latimer KS, Stenffens WL, Pesti D, and Lukert PD. Hemagglutination by psittacine beak and feather disease virus and use of hmagglutination inhibition for detection of antibodies against the virus. Am J Vet Res 52: 1810-1815, 1991a.
  70. Ritchie BW, Niagro FD, Latimer KS, Steffens WL, Pesti D, Campagnoli RP, and Lukert D. Antiboby response to and maternal immunity from and experimental psittacine beak and feather disease vaccine. Am J Vet Res 53: 1512-1518, 1992.
  71. Ritchie BW. Circoviridae. In: Avian viruses: fuction and control. Wingers Publishing, Inc. USA. 223-251, 1995.
  72. Ritchie BW and Harrison GJ. Viruses. In: Avian medicine: principles and application. Abridged ed. Wingers Publishing, Inc., Florida, USA, 894-902, 1997.
  73. Soike D. Circovirus infections in pigeons. Tierärztliche Praxis 25: 52-54, 1997.
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