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  • 學位論文

在活化的T 細胞中探討魚針草、刺五加及決明子的萃取物對細胞激素調節之影響

The Effects of Anisomeles Indica、Eleutherococcus Senticosus and Cassia Tora Extracts on Activated T cell-Mediated Cytokines

指導教授 : 廖雅芳
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摘要


活化的T 淋巴細胞在細胞介導的自體免疫性疾病和慢性炎症性疾病的發病機制中扮演關鍵角色。活化的T 細胞受體/ Cluster of differentiation 3 (CD3)複合物刺激受體CD28 訊息導致細胞激素如Interleukin-2(IL-2)、IL-4 及IL-13 的表現。轉錄因子活化T 細胞核因子(Nuclear factor of activatedT cells,NFAT)和核因子-κB(Nuclear factor-κB,NF-κB)對於這些細胞激素的表現發揮了重要調節作用。許多文獻指出Phorbol myristate acetate(PMA)和Ionomycin 可被用來模擬T 細胞的活化,因此本研究以這樣的方式活化T 細胞。魚針草(Anisomeles indica)具有抗人類免疫缺陷病毒(Human immunodeficiency virus,HIV)、抗幽門螺旋桿菌、清除自由基、抑制環氧合酶Ⅰ、抗組胺劑和抗痛覺過敏等活性。刺五加(Eleutherococcussenticosus)具有抗發炎、抗氧化、抗病毒活性、免疫保護和免疫調節作用。決明子(Cassia tora)則具有降血脂、降血壓、抑制組織胺釋放、抗血小板聚集、抗真菌、抗突變、抗氧化、保護肝臟、治療夜盲症、高血壓、高膽固醇血症和便秘的功能。然而,這些具有抗發炎的草本植物對於T 細胞的免疫反應之調節尚未清楚。因此,本論文在活化T 細胞中使用魚針草、刺五加及決明子等萃取物評估其對細胞激素之調節。研究發現在活化JK 細胞中魚針草萃取物降低NFAT 和NF-κB 轉錄活性,並且抑制IL-2、IL-4 及IL-13的mRNA 的表現。綜合以上研究顯示魚針草萃取物透過降低轉錄因子NFAT和NF-κB 的活性,進一步抑制下游分子細胞激素IL-2、IL-4 及IL-13 的表現而達到免疫抑制的效果;刺五加萃取物則未具有抑制作用,推斷刺五加萃取物可能透過影響其他免疫細胞的反應,而達成免疫抑制作用;決明子萃取物則是能增加細胞激素的表現,而達到對T 細胞的免疫刺激作用。 關鍵字:T細胞活化、細胞激素、魚針草、刺五加、決明子

並列摘要


Activated T lymphocytes play a pivotal role in the pathogenesis of cell-mediated autoimmune diseases and chronic inflammatory disorders. T cell triggered by the activation of the T cell receptor/ cluster of differentiation 3 (CD3) complex and costimulatory receptor CD28 leads to the expression of cytokines such as interleukin-2 (IL-2), IL-4 and IL-13. Transcription factors nuclear factor of activated T cells (NFAT) and nuclear factor-kappa B (NF-κB)play essential roles in regulating the cytokines expressions. Many studies report that phorbol myristate acetate (PMA) and ionomycin can be used to simulate T cell activation. Anisomeles indica (AI) has anti-human immunodeficiency virus (HIV) anti-Helicobacter pylori, free radical scavenging, cyclooxygenase I inhibitory, anti-histamine, analgesic and anti-hyperalgesic activities. Eleutherococcus senticosus (ES) has anti-inflammatory, antioxidant, antiviral activity, immune protection and immune regulation. Cassia tora (CT) is associated with lowering blood pressure, inhibiting histamine release , anti-platelet aggregation, anti-fungal, anti-mutagenic, anti-oxidation, and protecting liver damage, night blindness,hypertension, hypercholesterolemia and constipation. However, the effect of these herbs on T cell immunity are still unclear. Therefore, this study investigate the effects of AI, ES and CT extracts on T cell activation. We found that AI extract decreased NFAT and NF-κB transcriptional activities.Furthermore, it inhibited the mRNA expressions of IL-2, IL-4 and IL-13 in activated JK cells. ES and CT extracts could not repress the transcription factor activities. ES extract also did not decrease the cytokines expressions in activated JK cell. However, CT extract promoted the cytokines expressions in activated JK cell. Based on the above data, we suggest that AI inhibits IL-2,IL-4 and IL-13 mRNA expressions by reducing the activity of NFAT and NF-κB to provide the immunosuppressive activity. ES has not the inhibited effect in activated T cells. It has the immunosuppressive activity, possibly by inhibiting other immune cell actions. CT could increase the cytokines expression to achieve the immune stimulatory effect on T cells. Keywords: T cell activation, Cytokine, Anisomeles Indica, Eleutherococcus Senticosus, Cassia Tora

參考文獻


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