微量固相萃取結合液相層析串聯質譜術於老鼠血清中氫氧化四甲基銨之檢測

林佳穎

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微量固相萃取結合液相層析串聯質譜術於老鼠血清中氫氧化四甲基銨之檢測

Determination of tetramethylammonium hydroxide in mouse serum by micro solid-phase extraction combined with liquid chromatography tandem mass spectrometry

林佳穎 , Masters　　Advisor：李茂榮　　

繁體中文

氫氧化四甲基銨；微量固相萃取；基質效應；液相層析串聯質譜術； tetramethylammonium hydroxide ； µ ； SPE ； matrix effect ； LC-MS/MS

Abstract │ Reference (52) │ Times Cited (1) │ Altmetrics

Abstract 〈TOP〉

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A simple and sensitive method using micro solid-phase extraction (&micro;SPE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for determination trace amount of tetramethylammonium hydroxide (TMAH) in mouse serum was evaluated in this thesis. The heated electrospray ionization (HESI) with positive mode of triple quadrupole mass spectrometry was used to analyze tetramethylammonium hydroxide. Tetramethylammonium was extracted from serum by &micro;SPE with a mixed-mode cation exchange sorbent and 0.1% trifluoroacetic acid in acetonitrile used as the eluting solvent. The volume of sample used was 15 &micro;L. The linear range of the method was 10-2500 ng/mL with coefficient of determination at 0.9944. The limit of detection and limit of quantitation was 1.9 and 6.3 ng/mL, respectively. The intra-day and inter-day precisions with relative standard deviations were less than 2.5 and 9.8 %, respectively. The recovery of the method was greater than 95.6 %. As the matrix effect was unconsidered from the results of post-column infusion and post-extraction spiked. The feasibility of applying the proposed method to determine tetramethylammonium in serum was examined by analyzing real mouse serum sample of TMAH-exposured. The concentration of tetramethylammonium detected was range from 1.1 &micro;g/mL to 9.1 &micro;g/mL. The results of this study reveal the proposed method offers low ng/mL level sensitivity and high selectivity for determination of tetramethylammonium in mouse serum.

Reference ( 52 ) 〈TOP〉

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連結：
3.勞工安全衛生研究所，工安警訊，“要命！勞工不慎遭氫氧化四甲基銨溶液噴濺中毒致死案例” (2007)
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6.K. Shiomi, M. Mizukami, K. Shimakura, Y. Nagashima, Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 107 (1994) 427−432
連結：
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Times Cited (1) 〈TOP〉

蘇筠婷(2015)。分散式固相萃取技術結合高效能液相層析串聯質譜術於人體尿液中精氨酸及其衍生物之檢測。中興大學化學系所學位論文。2015。1-68。

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