以銨根電極為基礎研製固態式酵素生醫感測器

Study and Fabrication on Solid State Enzyme Biosensors Based on Ammonium Ion-Selective Electrodes

鄒年烜(Nien-Hsuan Chou)

指導教授 ： 熊慎幹

中原大學/工學院/電子工程研究所/碩士(2004年)

https://doi.org/10.6840/cycu200400372

摘要

尿素在人體尿液之衰減及血液中尿素濃度的升高係判斷腎功能的重要指標，故尿素之檢驗和測定係生物醫學和臨床分析應用上的重要參數。雖然有許多方法可進行尿素之檢測，如分光光度計法及直接分光譜測量之方法，酵素法是選擇性較佳及廣泛使用之方法。
本論文係利用將尿素?固定於銨根離子選擇電極表面以製作固態電位式尿素感測器。銨根離子選擇電極係以二氧化錫/氧化銦錫玻璃為基板，而尿素酵素係固定於混合含羰基之聚氯乙烯與無活菌素之的薄膜上。另一方面，本論文係藉由以Nicolsky-Eisenman方程式為基礎之實驗方法進行選擇係數之量測，且就尿素感測器對於主要待測離子及干擾離子間之選擇性進行評估。
依據研究結果得知此尿素感測器之響應時間快，量測環境之pH範圍係介於pH 6.0至pH 7.5間，於線性量測範圍(0.026 mM ~ 10 mM間)之感測度為55.56±3.15 mV/decade，最小量測極限為5 μM，其線性量測範圍內元件之感測度為58-61 mV/decade。以銨根電極為基礎所製作之尿素感測器，其優點為製程簡單、有良好之再線性及穩定性、低成本及架構可進行微小化。

關鍵字

含羰基之聚氯乙烯 ； 包埋法 ； 電位式固態尿素生醫感測器 ； 銨根離子選擇電極 ； 選擇係數

並列摘要

The detection and determination of urea are the important parameters and great interests in biomedical and clinical analysis applications where a reduced level in urine and an increased concentration in blood are the important indication for renal dysfunction. Although there are direct spectrophotometric methods of urea determination, enzymatic methods are more selective and much more widely used.
A potentiometric solid-state urea biosensor prepared by immobilization of urease directly onto the surface of an ammonium ion-selective electrode is described in this paper. The substrate of the ammonium ion-selective electrode is the tin oxide (SnO2) / indium tin oxide (ITO) glass and the enzyme was immobilized by entrapment method on a nonactin membrane incorporated carboxylated polyvinylchloride (PVC-COOH). However, for the quantitative expression for their selectivity is desired, the article employed the selectivity coefficients approach to estimate the selectivity of urea biosensors. In this study, experimental procedures based on the Nicolsky-Eisenman equation were considered. Therefore, the level of influence of the preference of the urea biosensors on the primary ions relative to interfering ions of is determined.
According to this paper, urea biosensors based on ammonium ion-selective electrodes respond rapidly and stable within the pH range from pH 6 to pH 7.5. The slope in the linear range (0.026 mM to10 mM) is about 55.56±3.15 mV/decade and the detection limit is 5 μM. Moreover, the use of ammonium ion-selective electrode has the advantage of providing solid-state urea biosensors that are easy to fabricate, good reproducibility and stability, low cost and allow miniaturization configuration.

並列關鍵字

entrapment method ； carboxylated polyvinylchloride (PVC-COOH) ； selectivity coefficient ； ammonium ion-selective electrode ； potentiometric solid-state urea biosensor

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