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Validation of a Field Monitoring System for BPU-Type IGR Resistance in the Diamondback Moth, Plutella xylostella-Presumed Working Scheme Based on IRAC Method No. 7 (Leaf Dipping Method)

應用IRAC Method No. 7(葉片浸藥法)偵測小菜蛾對BPU類生長調節劑抗性之研究

摘要


本研究係比較IRAC(Insecticide Resistance Action Committee)之建議方法,IRAC Method No. 7(葉片浸藥扶),與噴霧塔兩種方法測試小菜蛾對BPU類生長調節劑抗藥性之差異。葉片之沾藥量方面,葉片浸藥怯高出噴霧塔法3.29倍。葉片浸藥怯之測試時間為三齡蟲至化蛹,較一般噴霧塔方法之48小時為長,未發現小菜蛾對BPU類生長調節劑處理之葉片有拒食現象。由於葉片浸藥法採用較高的處理濃度與較長的測試時間,所得到之感度也比噴霧塔法更高。 以葉片浸藥法測試感性與抗性品系小菜蛾對chlorfluazuron、teflubenzuron、hexaflumuron及flufenoxuron四種BPU類生長調節劑感度,並以感性品系測試間隔六代之LC50差異,以確認此一方按之重現性。經以Indcpendent student's t-test分析,證實葉片浸藥怯適用於小菜蛾對BPU類生長調節劑抗藥性之檢測,感性品系小菜蛾與供試之3個抗性品系小菜娥LC50在95%之信賴區間有顯著差異,因而可檢出抗性。 四種供試之BPU類生長調節劑中,除hexaflumuron須以4~5倍之濃度間隔測試外,餘均以4至5個連續稀釋十倍之藥劑濃度測試,葉片浸藥法可有效檢測小菜蛾對BPU類生長調節劑之抗藥性。因IRAC Method No. 7係以餵食處理,不適用於BPU類生長調節添加協力劑piperonyl butoxid (PB)之測試。

並列摘要


Results of the IRAC Method NO.7 (leaf dipping method) were compared to those of the spray tower method in aspect of the resistance of diamondback moth (DBM), Plutella xylostella, to benzoylphenyl urea (BPU)-type insect growth regulators (IGR). The leaf dipping method resulted in 3.29-fold higher BPU-IGR deposit than the spray tower method. The treating period of leaf dipping lasts from 3rd instar to pupation, which is longer than the 48-hour period in the spray tower method. Both the higher dosage and the longer treating period of leaf dipping had resulted in higher sensitivity of the DBM to the treated doses of BPU-type IGR compared to the spray tower method. No anti-feeding effect of BPU-type IGR against DBM was observed. The susceptibility of a susceptible strain to 4 BPUs, chlorfluazuron, teflubenzuron, hexaflumuron and flufenoxuron, had been determined by using the leaf dipping method. The reproducibility of this method was tested and substantiated by comparing LC(subscript 50S) in the n and n+6 generations of the susceptible strain. The ability of leaf dipping method in detecting the BPU resistance in DBM was verified by the Independent Student's t-distribution test, which confirmed the separation of LC(subscript 50S) of the susceptible strain from 3 BPU-resistant strains at 95% fiducial limits. We concluded that four to five IGR concentrations in 10-fold increments with water are sufficient for testing BPU-resistance in DBM, except for hexaflumuron, the increment should be adjusted to 4-5 fold. The leaf dipping method is not suitable for synergistic with piperonyl butoxide (PB) due to different methods were applied to the DBM.

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