肉毒桿菌神經毒素注射咬肌對大白鼠顱顏生長發育之影響

Effects of an Injection of Botulinum Neurotoxin into the Masseter Muscle on Craniofacial Growth and Development in Rats

蔡志孟(Chih-Mong Tsai) ； 廖怡萱(Yi-Hsuan Liao) ； 楊良友(Liang-Yo Yang) ； 蔡吉陽(Chi-Yang Tsai)

《中華牙醫學雜誌（中文版）》 24卷4期 (2005/12) Pp. 231-239

https://doi.org/10.7106/CJD.200512.0231

摘要

本研究籍由將肉毒桿菌神經毒素注射入幼年大白鼠咬肌，造成咬肌萎縮，來探討咬肌對顱顏骨骼及生長發育的影響。研究方法是選取13隻年齡為30天的Wistar雄性大白鼠，分為實驗組10隻，將肉毒桿菌神經毒素施打入大白鼠一側咬肌，另一側施打生理食鹽水；對照組3隻大白鼠不施打任何藥劑。待大白鼠發育年齡為75天時將大白鼠犧牲，取下咬肌測量重量，及量測乾燥頭顱骨。結果發現實驗組大白鼠施打肉毒桿菌神經毒素側其咬肌重量較施打生理食鹽水側及對照組小，其顱顏骨型態在顧骨弓至下顎骨綠距離，冠狀突切跡最前端至下顎骨角狀突最下點距離，均小於生理食鹽水側距離，有統計上之差異。實驗結果顯示咬肌萎縮後，確實會影響顱顏骨型態及生長發育。

關鍵字

肉毒桿菌神經毒素 ； 咬肌 ； 肌肉萎縮 ； 顱顏骨骼 ； 生長發育

並列摘要

The purpose of this study was to evaluate the influence of the masseter muscle on craniofacial growth and development by injecting botulinum neurotoxin to produce masseter muscle atrophy. Ten male Wistar rats (30 days old) received a botulinum neurotoxin injection in the masseter muscle on 1 side and normal saline on the other side; another 3 rats received no injection and served as the control group. All rats were sacrificed at 75 days of age. The masseter muscles were dissected and weighed, and anthropometric measurements were made directly on dried rat skulls. The results showed that the masseter muscle injected with botulinum neurotoxin was significantly smaller than that without an injection. The craniofacial structures directly attached to the masseter muscle were significantly smaller on the side which received the botulinum neurotoxin injection compared to that which received a normal saline injection. Results of our study suggest that atrophy of the masseter muscle may alter craniofacial growth and development.

並列關鍵字

botulinum neurotoxin ； masseter muscle ； muscle atrophy ； craniofacial bone ； growth and development