The Interactions between Fish Nodavirus and Fish Retrovirus during Co-infection

魚類結病毒與魚類反轉錄病毒共感染寄主期間之交互作用研究

陳立涵(Li-Han Chen) ； 齊肖琪(Shau-Chi Chi)

《Acta Zoologica Taiwanica》 14卷2期 (2004/10) Pp. 115-116

https://doi.org/10.6576/AZT.2004.14.(2).13

摘要

SSN-1細胞株常被作爲魚類神經壞死症病毒(NNV)之研究工具，但有魚類反轉錄病毒SnRV的持續性感染。Lee等(2002)將SSN-1細胞上清液感染另一株對NNV有感受性的細胞株GF-1，成爲SnRV持續性感染的SGF-1細胞，以便研究SnRV與NNV共感染期間的交互作用。本實驗目的在於詳細探討共感染期間SnRV與NNV的交互影響。實驗結果顯示，SnRV的反轉錄酶(RT)會將NNV的兩段單股正意RNA皆以高相似度轉錄爲cDNA，即便在NNV的核酸序列上找不到SnRV RT可以辨識結合的序列。觀察NNV與SnRV共感染期間的日變化，發現NNV cDNA第三天才能測到，SnRV的RNA則在第四天就完全測不到，上清液中的NNV以GF-1與SGF-1兩種細胞定力價，發現第三天到第五天兩系統測出的力價有最大差異，因此第三天是所有變化的重要時刻，詳細推論將於正文中說明。

關鍵字

結病毒 ； 共感染 ； 神經壞死症病毒NNV ； 交互作用

並列摘要

SSN-1 cell line was used for many research of NNV, but it was SnRV-persistently infected. Lee et al. (2002) infected GF-1, which could also be infected by NNV, with the supernatant of SSN-1, and then got a SnRV-persistently infected cell line called SGF-1. Next, SGF-1 was used to investigate the interactions of the viral co-infection. The goal of this research was to study the interactions between NNV and SnRV during co-infection. The experiment revealed that the RT of SnRV reverse transcripted both positive singlestrand RNAs of NNV to high identified cDNA, but there was no primer binding site for SnRV RT recognizing in the RNA of NNV. To observe the daily change of co-infection, I found that the cDNA of NNV could be detected from the 3rd day, and the RNA of SnRV could not be detected after the 4th day. In addition, the titers of NNV in the supernatant during the 3rd to 5th day were much different between detected with GF-1 and SGF-1. Therefore, I supposed that the third day after NNV infecting was the important point of the change. The details of discussions were in the article.

並列關鍵字

nodavirus ； co-infection ； nervous necrosis virus NNV ； interaction