以超解析率明視野光學顯微術觀測癌細胞絲狀偽足的動態變化

Dynamics of Cancer cell Filopodia Characterized by super-resolution bright-field Optical Microscopy

李超煌(Chau-Hwang Lee) ； 廖唯昱(Wei-Yu Liao) ； 許慈軒(Tsi-Hsuan Hsu)

《光學工程》 101期 (2008/03) Pp. 68-78

https://doi.org/10.30011/OE.200803.0007

摘要

許多文獻指出，位於細胞邊緣，由肌動蛋白絲(actin filament)所組成直徑約為100-300nm的絲狀偽足(filopodium)和癌細胞移動能力有高度相關性。本論文藉使用有奈米縱向解析率的超解析率非干涉式廣視野光學測繪術(supre-resolution NIWOP)來觀測直徑小於光學解析率的絲狀偽足，我們使用此系統觀測不同的作用因子、基因或是藥物濃度梯度對於肺腺癌細胞絲狀偽足的影響，進而推斷是否會促進癌細胞的轉移。

關鍵字

肺腺癌細胞 ； 絲狀偽足 ； 超解析率非干涉式廣視野光學測繪術 ； 影像重建運算

並列摘要

In this thesis, we explore the dynamics of cellular filopodia with diameters around 200nm by using super-resolution bright-field optical microscopy. Filopodia are positively related to cell motility because of the low optical contrast of filopodia, we use an optical technique with nanometer depth sensitivity, non-interferometric widefield optical profilometry (NIWOP), to obtain the high contrast signal for super-resolution image restoration. The image contrast of NIWOP comes from the topographic variations of sample, rather than fluorescence labeling. With super-resolution NIWOP, we can easily observe the effect on filopodia by the treatment of various factors, genes or chemical gradient of drug and then conjecture that if they can enhance or suppress cancer-cell migration and invasion.

並列關鍵字

CL1-0 ； Filopodia ； Super-resolution NIWOP