黑木耳與維他命E對預防以飲食誘導實驗兔之動脈硬化的效果

The effect of Auricularia polytricha and Vitamin E on the prevention of diet-induced atherosclerosis in rabbits

黃萱雯(Hsuan-Wen Huang)

指導教授 ： 蔡清恩

國立屏東科技大學/農學院/獸醫學系所/碩士(2012年)

https://doi.org/10.6346/NPUST.2012.00235

摘要

動脈粥狀樣硬化 ( atherosclerosis ) 是引起心肌梗塞、缺血性心肌病或腦中風等疾病的主因。因此從日常飲食來控制動脈粥狀樣硬化的程度備受矚目。其中黑木耳 ( Auricularia polytricha ) 為普遍的食材，也應用於傳統藥材，其含豐富的多醣體，且在近幾年研究中指出具有許多生物活性，包含抗氧化、抗凝血和降低血糖等。而vitamin E為人體所需的營養素之ㄧ，有文獻指出其在高膽固醇飲食動物雖無法降低其組織和血漿中膽固醇，但其具有抗氧化功能，可以減緩LDL氧化或者減少巨噬細胞傷害血管內膜和減緩細胞激素引起的細胞反應。因此本實驗的設計原理在模擬日常生活高脂高膽固醇飲食下，以黑木耳的生物活性效用加上vitamin E 抗氧化作用，應用於預防飲食誘導兔子動脈硬化的效應，以探討在預防動脈硬化等心血管疾病方面是否具有加成的效果。實驗結果顯示，黑木耳和vitamin E在單獨使用時具有降血脂和抗動脈硬化之效果，但當兩者合併使用時，反而會導致血脂下降程度較單獨使用時來得少且使動脈粥狀樣硬化的病灶更顯著，因此推測這兩種物質可能會互相影響而造成其抗氧化或降血脂的機制，因此不適於合併在一起使用。

關鍵字

黑木耳 ； 維他命E ； 動脈粥狀樣硬化 ； 高血脂 ； 抗氧化 ； 膽固醇

並列摘要

Hyperlipidemia is a dangerous factor to cause atherosclerosis so that serum lipid level control is a target for prevention or treatment of atherosclerosis. Auricularia polytricha is one of the universal food materials, it contains the rich polysaccharide body. It possesses many biological activities, such as antioxidant, anti-hemoglutination and anti-high blood sugar level. Vitamin E is a kind of nutrition for health, it has antioxidant effect, may slow down the LDL oxidation, and may reduce macrophage content in the injured vascular itima and inhibit cytokine-induced cell response. The purpose of this study was designed a high-fat and high-cholesterol diet contained a combination of Auricularia polytricha with vitamin E to investigate if they can be as prevention of atherosclerosis progress in rabbits for the synergistic effect. During the experiment, we recorded the body weight, and measured the LDL-C, HDL-C, triglyceride, cholesterol in rabbit serum to compare the different between each of groups. The results indicated that the high-fat diet groups had significantly different with control group, it demonstrated that this animal model of high serum lipid was induced successfully, and the serum lipid level decreased when used Auricularia polytricha or vitamin E alone. But when two materials were used together, the lipid level was not significant decrease in serum compared to use Auricularia polytricha or vitamin E alone. The ascending aorta was collected to study for atherosclerosis lesion by light microscopy and SEM. The results showed the Auricularia polytricha combined with vitamin E administrated in rabbits didn’t have synergistic effect in prevention of atherosclerosis progress.

並列關鍵字

Auricularia polytricha, ； vitamin E ； atherosclerosiss ； hyperlipidemia ； anti-oxidation ； cholesterol

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