The study aimed to investigate the gap between expected and actual mothers’ parenting for teenaged girls and the impact on mother-daughter relationship. A total of 649 high school girl students responded to the questionnaire, inclusive of the two parts: mother-daughter relationship scale measurement and mothers’ parenting scale measurement. Then, the results were processed and analyzed by descriptive statistics, t-test, one-way ANOVA, simple linear regression analysis and multiple stepwise regression analysis. The major findings of the study were as follows: 1. Young girls’ whole perceptions on their mother-daughter relationship scored high. The data based on the scale measurement further showed that the part of the sense of attachment scored the highest and the part of the negative affect scored the lowest. 2. The scores of the practical and expected mothers’ parenting of young girl daughters both presented high. To further analyze, the expected mothers’ parenting generally scored higher than the practical mothers’ parenting. However, only the aspects of mothers’ behaving models and their attitudes toward daughters’ grades in the two items of “love and care” and “nurture of daughters” showed the opposite results. 3. From the results of the measurement of the practical mothers’ parenting, the aspect of “care for life” scored the highest, whereas that of “economical support” scored the lowest. From the results of the measurement of the expected mothers’ parenting, the aspect of “care for life” scored the highest, whereas that of “nurture of daughters” scored the lowest 4. The result showed significantly different on the scores of “love and care, ” “care for life,” “development of daughters, ” “economical support” in the part of the gap between expected and actual mothers’ parenting. 5. Due to mothers’ different levels of education, the result showed significantly different on the scores of “the whole aspects, ” “nurture of daughters, ” “development of daughters, ” “economical support” in the part of the practical mothers’ parenting. Meanwhile, the result showed significantly different on the scores of “the whole aspects, ” “love and care, ” “development of daughters, ” “economical support” in the part of the expected mothers’ parenting. 6. Due to mothers’ dedication to the working field or not, the result showed significantly different on the scores of “care for life” and “economical support” in the part of the practical mothers’ parenting. As for the part of the expected mothers’ parenting, the result showed significantly different on the scores of “economical support. ” 7. Mother-daughter relationship can be effectively predicted by the idea of teenaged girls’ perceptions on the practical mothers’ parenting. In addition, the item of “love and care” is the main variable when predicting mother-daughter relationship. 8. The result showed significantly different on the scores of Mother-daughter relationship when there’s different for teenaged girls’ perceptions on the expected and practical mothers’ parenting. 9. Mother-daughter relationship demonstrates the best when there’s no difference for teenaged girls’ perceptions on the expected and practical mothers’ parenting. Based on the findings above, the study offered further discussion and suggestions for future studies and application.